山羊脑多头蚴线粒体nad1基因的克隆及序列分析  

Cloning and Sequence Analysis of Mitochondrial nad1 Gene for Coenurus cerebralis on Goats in Hunan Province

作　　者：汤国祥 刘笛秋 

机构地区：[1]湖南省益阳市赫山区畜牧水产局,湖南益阳413000

出　　处：《中国畜牧兽医》2011年第9期87-90,共4页China Animal Husbandry & Veterinary Medicine

基　　金：长沙科技计划一般项目(K0902144-21)资助

摘　　要：本研究旨在阐明脑多头蚴湖南分离株线粒体烟酰胺腺嘌呤二核苷酸(NADH)脱氢酶亚单位1基因(nad1)部分序列(pnad1)的遗传变异情况,并用pnad1序列重构脑多头蚴与其他带科绦虫的种群遗传关系。利用聚合酶链反应(PCR)扩增脑多头蚴的pnad1,应用ClustalX 1.81程序对序列进行比对,再用Phylip3.67程序MP法和Mage4.0程序NJ法绘制种系发育树,并用Puzzle5.2程序构建最大似然树,同时利用DNAStar 5.0中的Megalign程序进行同源性分析。结果显示,所获得的pnad1序列长度分别均为430bp,湖南分离株与已知多头带绦虫位于同一分枝。由于脑多头蚴pnad1序列种内相对保守,种间差异较大,故均可作为种间遗传变异研究的标记,从而为脑多头蚴的分子流行病学和其相关疾病的诊断奠定基础。The objectives of the present study were to examine sequence variation in the mitochondrial NADH dehydrogenase subunit 1（nad1）gene among Coenurus cerebralis isolates in Hunan province,and to reconstruct their phylogenetic relationship using nad1 sequences.The partial nad1（pnad1）was amplified from each C.cerebralis,and pnad1 sequences were aligned using the ClustalX 1.81.MP and NJ trees of pnad1 were constructed using the software Phylip 3.67 version 4.0 and Mage version 4.0,and ML tree was also constructed using Puzzle version 5.2.sequence homology analysis was performed using the Megalign program of the software DNAStar version 5.0.The results showed that the lengths of pnad1 sequences were 430 bp.The constructed phylogenetic tree revealed that the Hunan isolates and the Taenia multiceps available in GenBank were clustered in the same clade.There is no significant variation in pnad1 sequences within C.cerebralis,while inter-species difference is obvious.It is concluded that pnad1 sequences can be used as genetic marker for population genetic studies of cestodes.The results of the present study provided foundation for further studies of molecular epidemiology of C.cerebralis,and for diagnosis of the resultant disease.

关 键 词：脑多头蚴 线粒体DNA nad1基因 种系发育关系 

分 类 号：Q78[生物学—分子生物学]