禽网状内皮组织增殖病病毒GD09株的分离与全基因组测序  

Isolation and the Full-length Genome Sequencing of Avian Reituculoendotheliosis Virus GD09 Isolate

作　　者：史伟伟[1] 刘红波[1] 徐成刚[1] 廖明[1] 曹伟胜[1] 

机构地区：[1]华南农业大学兽医学院农业部动物疫病防控重点开放实验室,广东广州510642

出　　处：《中国畜牧兽医》2011年第9期170-174,共5页China Animal Husbandry & Veterinary Medicine

基　　金：广东省科技计划项目(2010B020307007;2009A02010-1006);农业公益性行业科研专项(200803019)

摘　　要：从广东某鸡场送检的疑似禽网状内皮组织增殖病的病鸡中采集病料,处理后经接种DF-1细胞,经聚合酶链式反应(PCR)及特异性间接免疫荧光试验(IFA),分离并鉴定出1株禽网状内皮组织增殖病病毒(reticuloendotheliosis virus,REV),命名为GD09。依据已发表的REV前病毒全基因组序列设计并合成6对引物,采用分段扩增的方法完成了分离株的全基因组序列测定。结果显示,该分离株基因组序列全长8295bp,符合典型的复制完全型反转录病毒的基因组结构,基因序列中不含已知致癌基因。将该分离株的亚群特异性gp90基因序列与国内外各参考株相应序列进行相似性比较分析,结果表明,GD09分离株与中国分离株HA9901亲缘关系最近,核苷酸相似性最高。An avian reticuloendotheliosis virus（REV）,designated as GD09,was isolated and identified by DF-1 cell culture,PCR and immunofluorescence assay specific to REV from broiler breeder suspected with REV infection in Guangdong province.Based on the public full-length proviral genome sequence of avian reticuloendotheliosis virus strain,six pairs of primers were synthesized,and the full-length proviral genome of GD09 isolate was sequenced by the method of fragment amplification.The full-length proviral genome sequence of GD09 isolate was 8295 bp;its size had a little difference from that of published full-length genome sequences,but its structure corresponded with typical retroviral genome structure for completely copy ability,the known oncogenes were not included in its genome.According to the gp90 gene sequence comparison of GD09 strain with those of the other REV strains at home and abroad,the sequences of gp90 gene of GD09 strain and phylogenetic analysis showed that the gp90 gene of GD09 strain had the closest genetic relationship to HA9901 strain.

关 键 词：禽网状内皮组织增殖病 禽网状内皮组织增殖病病毒 gp90 分离与鉴定 

分 类 号：S858.31[农业科学—临床兽医学]