舒巴坦、利血平、乙二胺四乙酸二钠对鸭源大肠杆菌抗菌药物敏感性的影响  

Effects of Sutlbactam,Reserpine,EDTA to Duck Source Escherichia coli Antimicrobial Sensitivity

作　　者：刘建华[1] 苑丽[1] 胡功政[1] 潘玉善[1] 吴华[1] 陈君培[1] 

机构地区：[1]河南农业大学牧医工程学院,河南郑州450002

出　　处：《中国畜牧兽医》2011年第9期178-181,共4页China Animal Husbandry & Veterinary Medicine

基　　金：国家自然科学基金项目(31072170)

摘　　要：本试验旨在研究β-内酰胺酶抑制剂舒巴坦、外排泵抑制剂利血平及膜通透促进剂乙二胺四乙酸二钠(EDTA)对鸭源大肠杆菌抗菌药物敏感性的影响。采用微量肉汤稀释法测定8株多重耐药鸭源大肠杆菌对头孢噻呋钠、恩诺沙星、阿米卡星、氟苯尼考、多西环素及其与舒巴坦、利血平和EDTA联用的最小抑菌浓度(MIC)。结果表明,舒巴坦能使5株鸭源大肠杆菌对头孢噻呋钠的MIC值下降4倍以上;利血平仅能使3株菌株对头孢噻呋钠的MIC值下降4倍以上;而EDTA能使7、5、3、6、8株菌对以上5种药物的MIC下降4倍以上;舒巴坦+利血平使4株菌株对头孢噻呋钠的MIC下降4倍以上,使5株菌株对氟苯尼考的MIC下降4倍以上;舒巴坦+EDTA使鸭源大肠杆菌对以上5类药物的MIC大幅度下降,分别使8、5、2、8、8株菌MIC下降4倍以上;利血平+EDTA使7株菌株对恩诺沙星MIC下降4倍以上,使所有分离株对其余4种药物的MIC显著下降;舒巴坦+利血平+EDTA和5类药物联用后,所用分离株的MIC下降4倍以上。由此可见,引起鸭源大肠杆菌对以上抗菌药物的耐药原因主要包括细胞膜通透性的下降、药物的主动外排作用和产生β-内酰胺酶的破坏作用,三者相协同提高了鸭源大肠杆菌的耐药水平。To explore the relationship between antimicrobial-resistance phenotypes and the destruction of β-lactamases,the active efflux system,cell membrane permeability,observed the change of duck source E.coli antimicrobial sensitivity with or without Sulbactam,Reserpine,EDTA.The minimal inhibitory concentrations（MIC） of Ceftiofur sodium,Enrofloxacin,Amikacin,Florfenical,Doxycycline with or without Sulbactam,Reserpine,EDTA to duck source E.coli were determined by broth microdilution.There were separately five strains sensitivity increase significantly when Sulbactam combine with Ceftiofur sodium;while Reserpine had apparent effect on three strains to Ceftiofur sodium.EDTA had obvious function on the strains to all antimicrobial,there were 7,5,3,6,8 strains showed positive separately;when used Sulbactam and Reserpine at the same time,five strains sensitivity increased significantly if combined with Ceftiofur sodium and four with Florfenical.The isolates were significantly positive after added Sulbactam,Reserpine,EDTA simultaneously and five categories antimicrobial of MIC decreaseed obviously.The reason of duck source E.coli antibiotic resistance contains changes in cell membrane permeability,the active efflux system and the destruction role of β-lactamases,which combined improved the resistance level of duck source E.coli.The change in cell membrane permeability was the most important role in this study.

关 键 词：鸭源大肠杆菌 多重耐药 Β-内酰胺酶抑制剂 主动外排泵 膜通透性 

分 类 号：S852.62[农业科学—基础兽医学]