雷公藤内酯醇对人口腔鳞状细胞癌细胞株Tca8113作用的实验研究  

Effect of triptolide on human oral squamous cell carcinoma cell line Tca8113

作　　者：吴也可[1] 宋东哲[1] 景欢[1] 王馨玉[1] 赵振刚[1] 吕胡玲[1] 廖爽[1] 陈谦明[1] 

机构地区：[1]口腔疾病研究国家重点实验室,四川大学成都610041

出　　处：《国际口腔医学杂志》2011年第5期524-526,530,共4页International Journal of Stomatology

基　　金：四川大学大学生创新实验计划基金资助项目(2009459)

摘　　要：目的观察雷公藤内酯醇对人口腔鳞状细胞癌细胞株Tca8113的抑制作用,并与传统化疗药物顺铂的作用相比较,评价其作为抗头颈部恶性肿瘤药物的价值。方法噻唑蓝比色法检测雷公藤内酯醇和顺铂对Tca8113增殖的抑制作用,形态学观察经药物作用后细胞的生长情况。结果雷公藤内酯醇对Tca8113的增殖有明显抑制作用,呈量-效、时-效关系;较小浓度的雷公藤内酯醇即可产生较好的抑制效果,作用小于7 d时与顺铂相当,7 d时0.001 mg.L-1抑制率达60%,0.01 mg.L-1抑制率达90%,显著优于顺铂(P<0.05);1、3、5 d作用点时,在大于0.1 mg.L-1时顺铂效果优于雷公藤内酯(P<0.05),7 d时两者差异无统计学意义;各个作用点雷公藤内酯醇的50%抑制浓度均小于顺铂。结论雷公藤内酯醇对人口腔鳞状细胞癌细胞株Tca8113有明显的抑制作用,呈量-效、时-效关系,且在较低浓度和较长时间作用下其抑制率明显高于顺铂。Objective To evaluate the value of triptolide as an antitumor drug by observing its inhibitive effect on human oral squamous cell carcinoma（cell line Tca8113）,and to compare it with conventional chemotherapeutics cisplatin.Methods Methyl thiazolyl tetrazolium（MTT） assay was used to examine the inhibitive effect of triptolide on the proliferation of Tca8113 in vitro.The morphology of the cells treated with triptolide was observed by inverted phase contrast microscope.Results Triptolide had significant effect on Tca8113 in a dose-dependent and time-dependent manner（P〈0.05）;Lower concentration of triptolide could exert considerable inhibitive effect, the inhibitive rate of 0.001 mg·L-1 and 0.01 mg·L-1 triptolide was 60% and 90% at 7 days,respectively statistically significantly better than those of cisplatin;while the concentration more than 0.1 mg·L-1,cisplatin was superior to triptolide in inhibiting the cells proliferation at 1,3,5 days（P〈0.05）;the 50% inhibitory concentration of triptolide was less than those of cisplatin at every time point.Conclusion Triptolide has significant suppressive effect on human oral squamous cell carcinoma cell line Tca8113 in dose-and time-dependent manner（P〈0.05）, and it works better than cisplatin at low concentrations with longer acting time.

关 键 词：雷公藤内酯醇 顺铂 人口腔鳞状细胞癌细胞株Tca8113 抗肿瘤作用 噻唑蓝 

分 类 号：R285.5[医药卫生—中药学]