黄连总生物碱对糖尿病大鼠血管内皮细胞的影响  

The effect of total alkaloids from Coptidis Rhizoma on vascular endothelium cells in diabetic rats

作　　者：张家富[1] 高家荣[1] 姜辉[1] 张睿[1] 

机构地区：[1]安徽中医学院第一附属医院药学部,合肥230031

出　　处：《中药药理与临床》2011年第4期24-26,共3页Pharmacology and Clinics of Chinese Materia Medica

基　　金：安徽中医学院临床科研基金;编号:2009LC1-009A

摘　　要：目的:观察黄连总生物碱对糖尿病大鼠血管内皮细胞的影响。方法:链脲佐菌素一次性腹腔注射复制糖尿病大鼠模型。给药组灌胃给予黄连总生物碱(50,100,200mg/kg)、格列齐特(20mg/kg),正常组和模型组给予等容量生理盐水,每天1次,连续8周。实验结束后,分光光度法检测大鼠血糖水平,放射免疫法测定6-酮-前列腺素(6-Keto-PG)F1α、血栓素(TX)B2、血管紧张素(Ang)Ⅱ的含量;ELISA法检测血管性血友病因子(vWF)、纤溶酶原激活物抑制剂(PAI)-1的水平;取胸主动环观察不同累积浓度的乙酰胆碱(Ach)对去甲肾上腺素(NE)引起的血管收缩的抑制率;免疫组化技术测定主动脉细胞间粘附分子(ICAM)-1蛋白的表达。结果:黄连总生物碱不仅能显著降低糖尿病大鼠血糖水平、血浆TXB2、AngⅡ、vWF、PAI-1含量,升高6-Keto-PGF1α的水平,改善糖尿病大鼠内皮依赖性舒张功能,同时还能有效抑制糖尿病大鼠主动脉ICAM-1蛋白的表达。结论:黄连总生物碱对糖尿病大鼠血管内皮细胞具有一定的保护作用。Objective： To observe the effect of vascular endothelium cells by extraction total alkaloids from Coptidis Rhizome（TCA） in diabetic rats.Methods： Experimental diabetic rats was induced by streptozotocin.All the therapeutic groups were treated with TCA（50,100,200 mg/kg） and the gliclazide（20mg/kg） respectively for 8 weeks.The blood sugar level were determinate by chromatometry.The 6-keto-prostaglandin（6-Keto-PG）F1α,thromboxan（TX）B2,angiotonin（Ang）Ⅱcontent were assayed by radioimmunity,thevon Willebrand disease factor（vWF）,plasminogen activator inhibitor（PAI）-1 level were determinate by enzyme linked immunosorbent assay.Thorac-ic aortic ring was employed to observe the inhibition by different concentration of acetylcholine（Ach） to constraction caused by norepinephrine（NE）,SP immunohistochemicae staining was employed to observe the expression of intercellular adhesion molecule（ICAM-1）.Results： TCA not only could decreased the blood sugar,TXB2,AngⅡ,vWF,PAI-1content,reduced the inhibition of vasodilator responses,step up 6-Keto-PGF1α activities,but also effectively prevented the expression of ICAM-1.Conclusion： TCA has a protect function on injury of vascular endothelium cells in diabetic rats.

关 键 词：黄连总生物碱 糖尿病 血管内皮细胞 

分 类 号：R285[医药卫生—中药学]