口服Ⅱ型胶原肽-霍乱毒素B亚单位复合物对胶原诱导性关节炎的影响  

Effect of oral administration of type Ⅱ collagen peptide-cholera toxin B subunit complex on collageninduced arthritis

作　　者：赵欣欣[1] 毕莹[1] 贾秀坤[2] 闵睿[1] 尹晓燕[1] 

机构地区：[1]中国医科大学附属第四医院风湿免疫科,沈阳110032 [2]中国医科大学附属第四医院检验科,沈阳110032

出　　处：《中华风湿病学杂志》2011年第9期634-637,I0005,共5页Chinese Journal of Rheumatology

基　　金：基金项目：辽宁省教育厅高等学校科研项目计划（2008808）

摘　　要：目的关节炎急性期胶原诱导性关节炎（CIA）小鼠口服Ⅱ型胶原免疫活性肽段（250—270））[CⅡ（250—270）]和霍乱毒素B亚单位（CTB）的共价复合物，观察其是否能产生免疫耐受。方法DBA／1小鼠分成I、Ⅱ、Ⅲ、Ⅳ组，I组为健康对照，Ⅱ、Ⅲ、Ⅳ组小鼠制成CIA。Ⅲ组小鼠于关节炎发病率达到100％后，灌胃CⅡ（250—270）-CTB共价复合物，Ⅳ组小鼠于初次免疫后第14日灌胃CII（250-270）与CTB的混合物。记录小鼠关节炎症评分及病理评分。关节炎发生率比较采用Fisher精确概率法，关节炎累计评分及组织病理累计评分采用方差分析。结果I、Ⅱ、Ⅲ、Ⅳ组的关节炎发生率分别为0、100％、100％和25％；关节炎累计评分均值分别为0、5．0±1．7、10．8±2．8和1．0±2．0；关节炎病理累计评分均值分别为0、16±8、32±13和7±6。结论CIA小鼠于免疫后的关节炎临床前期口服CⅡ（250-270）与CTB的混合物能抑制关节炎的发生及严重程度，关节炎急性炎症期口服CⅡ（250—270）-CTB共价复合物后可能使关节炎症加重。Objective The aim of this study is to investigate whether oral administration of collagen Ⅱ peptide （250-270） [C Ⅱ（250-270）] -cholera toxin B subunit （CTB） complex could effectively set up oral immune tolerance to collagen-induced arthritis （CIA） in mice. Methods DBA/1 mice were divided into Ⅰ , Ⅱ , Ⅲ and ivgroups. GroupⅠ was normal control group. Collagen type Ⅱ emulsified in Freund＇s complete adjuvant were injected to mice of groups Ⅱ , Ⅲ and Ⅳ twice from the base of the tail. Mice of group Ⅲ were fed with C Ⅱ （250-270）-CTB covalent complex twice after the arthritis was developed. Mice of group Ⅳ were fed with C Ⅱ （250-270） and CTB mix at the 14th day after primary immunization. Visual scores and histopathologic scores of arthritis were recorded. The frequencies of arthritis between the groups were compared using Fisher＇s exact test. The clinical and histological severity of arthritis were analyzed by ANOVA. Results The frequencies of arthritis in groups I , Ⅱ, Ⅲand Ⅳ were 0, 100%, 100% and 25% respectively. Average accumulative scores of arthritis were 0, 5.0±1.7, 10.8±2.8 and 1.0±2.0 respectively. Average accum-ulative histopathological scores of arthritis were 0, 16±8, 32±13 and 7±6 respectively. Conclusion Oral administration of C II （250-270） and CTB mix in arthritis mice after C Ⅱ immunization can suppress the onset and severity of arthritis. Oral administration of C Ⅱ （250-270）-CTB covalent complex in the acute stage of arthritis can accelerate arthritis.

关 键 词：关节炎 实验性 免疫耐受 胶原Ⅱ型 霍乱毒素 小鼠 

分 类 号：R392[医药卫生—免疫学]