猪轮状病毒Rotavirus A pig/China/NMTL/2009/G9P[23]株的分离与鉴定  

Isolation and identification of the porcine rotavirus isolate of Rotavirus A pig/China/NMTL/2009/G9P[23]

作　　者：时洪艳[1] 陈建飞[1] 王承宝[1] 张志榜[1] 石达[1] 冯力[1] 

机构地区：[1]中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室/猪传染病研究室,黑龙江哈尔滨150001

出　　处：《中国预防兽医学报》2011年第9期681-684,共4页Chinese Journal of Preventive Veterinary Medicine

基　　金：哈尔滨市攻关项目(2009AA6AN029)

摘　　要：A群轮状病毒(GAR)是引起人类和多种动物腹泻的主要病原体,病原的分离与鉴定是轮状病毒(RV)流行病学、病原学等研究的基础。本研究采用接种MA104细胞的方法,从内蒙古某猪场患病猪腹泻粪便中分离一株病毒,经3次蚀斑克隆纯化后,采用电镜观察、PCR鉴定和序列测定进行分析,结果表明该分离株为猪轮状病毒(PoRV)。致病性试验表明该分离株能够引起仔猪急性腹泻,RT-PCR扩增其VP4、VP6和VP7的基因节段并进行序列测定,按照A群RV的最新分类方法,确定该分离株的VP6、VP7和VP4基因型分别为I5型、G9型和P[23]型。因此,将该分离株命名为Rotavirus A pig/China/NMTL/2009/Q9P[23]。Group A rotaviruses are the main pathogens which cause diarrhea in human and animals.An virus was isolated from piglet with diarrhea in Inner Mongolia and purified 3 times in MA104 cell.The virus was identified to be porcine rotavirus by electron microscopic observation,PCR and sequencing analysis,designated A pig/China/NMTL/2009/G9P[23].The pathogenicity experiment showed that the virus caused acute diarrhea in piglet.According to the sequence analysis,the virus was classified as group A rotaviruses,the genotype of VP4/6/7 was belonged to P[23],I5,and G9,respectively.

关 键 词：猪轮状病毒 分离 鉴定 

分 类 号：S852.65[农业科学—基础兽医学]