荧光定量RT-PCR方法与兔体定型热试验对于检测猪瘟兔化弱毒疫苗的平行关系  

Parallel relationship between real-time RT-PCR and fever reaction method in rabbits for testing hog cholera lapinized virus vaccines

作　　者：葛叶[1,2] 张兴娟[1] 朱庆虎[2] 韩秋影[1] 王牟平[2] 李伟杰[2] 孙建宏[2] 仇华吉[1] 

机构地区：[1]中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室/猪传染病研究室,黑龙江哈尔滨150001 [2]中国农业科学院哈尔滨兽医研究所维科生物技术开发公司,黑龙江哈尔滨150069

出　　处：《中国预防兽医学报》2011年第9期699-703,共5页Chinese Journal of Preventive Veterinary Medicine

基　　金：国家科技支撑计划(2006BAD06A03)

摘　　要：为探讨荧光定量RT-PCR技术与传统兔体定型热试验对于检测猪瘟兔化弱毒疫苗的平行关系,本研究采用荧光定量RT-PCR方法与兔体定型热试验对5份猪瘟兔化弱毒细胞苗成品和6份半成品样品进行平行检测。结果表明,两种检测方法存在正相关性,最低105个病毒拷贝可以使家兔产生定型热反应,最低103个病毒拷贝可以使家兔产生轻热反应。荧光定量RT-PCR检测方法的检测过程仅需3.5 h,大大缩短了猪瘟疫苗的检测时间,该方法可以补充传统的兔体定型热反应用于猪瘟兔化弱毒疫苗半成品与成品的检验。To evaluate a parallel relationship between the real-time RT-PCR method and fever reaction method in rabbits,we detected 5 finished and 6 semi-finished products of hog cholera lapinized virus（HCLV） vaccines.The results showed that both methods had positive correlation,105 copies of HCLV were able to induce typical fever reaction and 103 copies induce moderate fever reaction in rabbits.Only 3.5 hours was needed for the real-time RT-PCR,indicating that the real-time RT-PCR could be an alternative to traditional fever reaction test for HCLV vaccine dosage determination in rabbits.

关 键 词：猪瘟兔化弱毒疫苗 荧光定量RT-PCR 兔体定型热试验 

分 类 号：S852.5[农业科学—基础兽医学]