自制方化湿液对湿阻证大鼠胃黏膜的保护作用及对表皮生长因子受体表达的影响  

Protective effect of dampness-releasing liquid on gastric mucosa and expression of epithelial growth factor receptor in rats with dampness retention syndrome

作　　者：薛晓倩[1] 黄学宽[1] 高宁[2] 刘鄂湖[2] 

机构地区：[1]重庆医科大学中医药学院,重庆401331 [2]第三军医大学药学院生药学教研室,重庆400038

出　　处：《第三军医大学学报》2011年第18期1928-1931,共4页Journal of Third Military Medical University

基　　金：重庆市卫生局科技项目(2009-2-143)~~

摘　　要：目的研究自制方化湿液对湿阻证大鼠胃黏膜保护作用及对表皮生长因子受体(epithelial growth factor re-ceptor,EGFR)表达的影响。方法将40只SD大鼠分为正常组,模型组,化湿液低、中、高剂量组,每组8只。正常组常规喂养,其余各组采用改进的环境加疲劳法制造湿阻证模型,连续6 d。造模成功后正常组和模型组予生理盐水灌胃,化湿液治疗组按低、中、高剂量(4、8、16 g/kg)灌胃治疗,每天1次,连续8 d。采用比色法检测血清中褪黑素(MT)、谷胱甘肽过氧化物酶(GSH-Px)及丙二醛(MDA)的含量变化,并用免疫组化方法观察胃黏膜EGFR表达情况。结果与正常组相比,模型组大鼠脾虚症状明显,胃黏膜有明显充血水肿,并有出血点及血块,且血清MT及GSH-Px含量降低[MT:(9.68±1.48),GSH-Px:(368.10±52.92),P<0.05],MDA含量增高[(19.43±3.49),P<0.05];免疫组化显示,模型组大鼠胃黏膜EGFR表达较正常组减少[灰度值(166.013±7.757),光密度值(0.025±0.193),P<0.05]。经化湿液治疗后,与模型组比较,血清MT及GSH-Px含量增高(P<0.05),MDA含量降低(P<0.05),大鼠胃黏膜EGFR表达依次增加(P<0.05)。结论自制方化湿液可明显改善湿阻证大鼠脾虚症状并对胃黏膜损伤有保护作用,其机制可能与增强机体抗氧化应激能力及提高胃黏膜EGFR的表达量有关。Objective To study the protective effect of dampness-releasing liquid on gastric mucosa and expression of epithelial growth factor receptor（EGFR） in rats with dampness retention syndrome（DRS）.Methods Forty SD rats were randomly divided into control group,model group,and low,medium and high dampness-releasing liquid dose groups（8 in each group）.Rats in control group were fed with routine rat chow.A DRS model was induced by modifying the environment and increasing fatigue in the other groups for 6 d.Then,rats in control and model groups received intra-gastric normal saline（NS）,while those in low,medium and high dampness-releasing liquid dose groups received dampness-releasing liquid at the dose of 4 g/kg,8 g/kg and 16 g/kg respectively,once a day for 8 d.Serum melatonin（MT）,glutathione peroxidase（GSH-Px） and malondialdehyde（MDA） levels were measured by colorimetry and expression of EGFR was detected by immunohistochemistry in different groups.Results The symptoms of spleen insufficiency,hyperemia and edema in gastric mucosa with hemorrhagic spots and clots were more significant in model group than in control group（P0.05）.The serum levels of MT and GSH-Px were lower and that of MDA was higher in model group than in control group after treatment with dampness-releasing liquid（9.68±1.48,368.10±52.92,19.43±3.49,P0.05）.Immunohistochemistry showed that the expression level of EGFR in gastric mucosa was lower and the OD value was significantly higher,while the serum levels of MT and GSH-Px were higher and that of MDA was lower in model group than in control group after treatment with dampness-releasing liquid（166.013±7.757,0.025±0.193,P0.05）.Conclusion Dampness-releasing liquid can significantly improve the symptoms of spleen insufficiency and protect gastric mucosa against injury by increasing the anti-oxidative capacity and expression of EGFR in gastric mucosa.

关 键 词：化湿液 湿阻证 胃黏膜 表皮生长因子受体 自由基损伤 

分 类 号：R285.5[医药卫生—中药学] R289.5[医药卫生—中医学]