细胞因子抗体芯片筛选脓毒症患者血清蛋白标记物  

Screening the serum protein biomarker of patients with sepsis by cytokine antibody chips

作　　者：石玉玲[1] 胡塔[2] 

机构地区：[1]广州军区总医院检验科,广东广州510010 [2]广州医学院临床医学院,广东广州510182

出　　处：《细胞与分子免疫学杂志》2011年第9期1010-1013,共4页Chinese Journal of Cellular and Molecular Immunology

基　　金：全军"十一五"课题基金资助项目(08Z009)

摘　　要：目的:应用细胞因子抗体芯片(CAC)分析脓毒症患者血清中炎性细胞因子表达的变化。方法:采用CAC技术平台,测定9例脓毒症患者(脓毒症组)和4例正常健康人(对照组)血清中79种细胞因子。以两组样本中每组至少有4个样品的杂交信号值超过400为有表达的蛋白,筛选出有表达的蛋白38个。应用芯片差异显著性分析(SAM)软件对38个有表达的蛋白进行差异表达分析。结果:筛选到胰岛素样生长因子结合蛋白-1(IGFBP-1)、肝细胞生长因子(HGF)、骨桥蛋白(Osteopotin)、胰岛素样生长因子结合蛋白-4(IGFBP-4)、干扰素诱导蛋白-10(IP-10)和B淋巴细胞趋化因子(BLC)等6个在脓毒症患者血清中高表达的细胞因子,以及血小板衍生生长因子-BB(PDGF-BB)、脑源性神经营养因子(BDNF)、巨噬细胞炎性蛋白(MIP-1β)、白细胞介素-8(IL-8)、表皮生长因子(EGF)和白介素-1β(IL-1β)等6个在脓毒症患者血清中低表达的细胞因子。聚类分析表明,上述这12个差异表达的细胞因子能够区分脓毒症和正常健康对照。结论:CAC是一种高效、快速的蛋白质组学技术,利用CAC从血清中筛选脓毒症相关蛋白分子标记物是可行的。AIM： To investigate the expression of inflammatory cytokines in patients with sepsis by cytokine antibody chips.METHODS： To screen the protein biomarker for rapidly diagnosing sepsis,79 cytokines in 9 cases of patients with sepsis and 4 cases of healthy control were measured using cytokine antibody chip.Analyse the signaling values of these 79 cytokines using SAM（significance analysis of microarray,SAM） software.RESULTS： By SAM analysis： insulin like growth factor binding protein-1（IGFBP-1）,epidermal growth factor（HGF）,osteopotin,insulin like growth factor binding protein-4（IGFBP-4）,interferon inducible protein-10（IP-10） and B-lymphocyte chemoattractant（BLC） were identified to be highly expressed in the sepsis while platelet-derived growth factor-BB（PDGF-BB）,brain-derived neurotrophic（BDNF）,macrophage inflammatory protein-1β（MIP-1β）,interleukin-8（IL-8）,epidermal growth factor（EGF） and interleukin-1β（IL-1β）lowly expressed.Cluster analysis of significantly expressed cytokines showed that the sepsis and the control formed distinctly separate groups.CONCLUSION： Antibody chips demonstrate a significant change in sepsis patients,screening the protein biomarker for rapidly diagnosing sepsis is feasible.

关 键 词：脓毒症 细胞因子抗体芯片 非监督聚类分析 芯片差异显著性分析 

分 类 号：R631.4[医药卫生—外科学]