人胚胎脊髓发育过程中神经营养因子3的表达及变化  

Expression of neurotrophin-3 in the human embryo spinal cord

作　　者：郭建辉[1] 杨金伟[1] 贺永胜[2] 高燕[2] 殷露玮[2] 冯忠堂[2] 李力燕[2] 

机构地区：[1]昆明医学院附属昆华医院,昆明650032 [2]昆明医学院神经科学研究所,昆昵650500

出　　处：《解剖学杂志》2011年第4期436-438,453,F0003,共5页Chinese Journal of Anatomy

基　　金：云南省自然科学基金（2004C0003R,2007C219M,2008ZC108M）

摘　　要：目的：探讨神经营养因子3（NT-3）在不同发育时期人胚胎脊髓中的表达变化及意义。方法：采用免疫组织化学和免疫印迹法对4周～7月人胚胎脊髓发育过程中NT-3的定位及定量进行研究。结果：NT-3在所检测的11个时段中均有表达，从第6周开始逐渐增加，至第9周时达高峰，之后下降并维持在一定水平，且以细胞突起和纤维表达最明显。结论：NT-3广泛分布于人胚胎脊髓不同发育时期的各种组织中，对胚胎期脊髓的发育起重要作用，并可能对脊髓神经干细胞的分裂、增殖及分化有积极的促进作用。Objective： To explore the expression change and its significance of neurotrophin-3 （NT-3） at different developmental stages of human embryonic spinal cord. Methods： Immunohistochemistry and Western blot were used to systematically study the localization and quantitation of NT-3 in the human embryonic spinal cord from the 4th week to the 7th month. Results： NT-3 positive protein was found in all the detected 11 time points, and concentrated on cell process and fiber. Along with the embryonal age the quantitation of NT-3 increased gradually from the 6th week, reached the peak in the 9th week, and then decreased and maintained at a certain level. Conclusion： NT-3 is widely distributed in human fetal spinal cord of different developmental stages and may play an vital role in the embryonic stage. NT-3 possibly has an positive role in the division, proliferation and differentiation of the spinal neural stem cells.

关 键 词：神经营养因子3 人胚胎 脊髓 发育 

分 类 号：R965[医药卫生—药理学]