槲皮素诱导人宫颈癌Hela细胞凋亡的作用  

Quercetin induces apoptosis of human cervical carcinoma Hela cells

作　　者：马朋[1] 宋晓冬[2] 闫苗苗[1] 牛新华[1] 于敏[1] 

机构地区：[1]滨州医学院药学院,烟台264003 [2]滨州医学院实验中心,烟台264003

出　　处：《解剖学杂志》2011年第4期462-464,475,共4页Chinese Journal of Anatomy

基　　金：山东省自然科学基金（ZR2009EM006）

摘　　要：目的：检测槲皮素体外对人宫颈癌Hela细胞的增殖抑制及凋亡诱导作用，并探讨线粒体在诱导凋亡机制中的作用。方法：采用酸性磷酸酶法检测细胞增殖抑制率；丫啶橙（AO）／溴化乙锭（EB）荧光染色观测细胞形态结构变化；流式细胞仪检测线粒体膜电位（△ψm）。结果：槲皮素对人宫颈癌Hela细胞有明显的增殖抑制作用，且呈浓度和时间依赖性。经槲皮素作用48h后，AO／EB染色可见细胞呈凋亡形态学改变。100μmol／L槲皮素作用Hela细胞24、48h后，与对照组相比线粒体膜电位下降。结论：槲皮素体外能抑制人宫颈癌Hela细胞生长，引起线粒体膜电位下降，诱导细胞凋亡。Objective： To investigate the cell growth inhibition and apoptosis induced by quercetin on human cervical carcinoma Hela cells and the role of mitochondrium membrane potential in the process of apoptosis. Methods： The cell growth inhibiting ratio of Hela ceils was assessed by the acid phosphatase assay. Morphologic changes of the quercetin-treated Hela cells were observed by fluorescence microscopy after acridine orange/ethidium bromide （AO/EB） staining. The cell mitochondrial membrance potential was detected by flow cytometry. Results： Quercetin inhibited the proliferation of Hela cells significantly, and the growth inhibitory rate presented time and dose-dependent relationship. Typical morphological changes of cell apoptosis were observed after the Hela cells had been exposed to quercetin for 48 hours by the using AO/EB staining method. The cell mitochondrial trans-membrance potentials in Hela cells was declined after being treated with 100 μmol/L quercetin for 24 and 48 hours compared with the control group （P〈0. 01）. Conclusion： Quercetin can inhibit the proliferation of Hela cells, and induce cell apoptosis by declining trans-membrance potentials of mitochondrium.

关 键 词：槲皮素 宫颈癌细胞 细胞凋亡 

分 类 号：R284.1[医药卫生—中药学]