低氧对大鼠海马神经元酪氨酸蛋白激酶Fyn表达的影响  

Expression of non-receptor tyrosine kinases-Fyn in the hippocampus of the rats exposed to hypoxia

作　　者：廖家万[1] 钟敬亮[1] 胡煜辉[1] 杨赣军[1] 胡红梅[1] 李小林[1] 袁琼兰[2] 

机构地区：[1]井冈山大学医学院解剖学教研室,吉安343300 [2]同济大学医学院解剖学与神经生物学教研室,上海200092

出　　处：《解剖学杂志》2011年第4期511-513,共3页Chinese Journal of Anatomy

基　　金：国家自然科学基金（30971531）

摘　　要：目的：探讨低氧对大鼠酪氨酸蛋白激酶Fyn表达的影响。方法：大鼠随机分为正常对照组和低氧组，低氧组大鼠进行6周常压缺氧饲养。低氧结束后，采用免疫组织化学、RT-PCR方法，分别观察大鼠海马的Fyn蛋白以及mRNA水平的变化。结果：Fyn在两组大鼠海马CA3区均有表达，与对照组相比，Fyn在低氧组大鼠海马CA3区辐射层表达比对照组降低；同时低氧组动物Fyn的mRNA水平降低。结论：低氧环境使大鼠海马中的Fyn蛋白及其mRNA表达均降低。Objective： To investigate the effect of hypoxia on the expression of Fyn in the hippocampus of rats. Methods： Adult male Wistar rats were randomly divided into control and hypoxia groups. Rats in the hypoxia group were exposed to hypoxia for 6 weeks. Expressions of the Fyn in the hippocampus of the rats were observed by immunohistochemical and RTPCR methods. Results ： The Fyn was expressed in CA3 of the hippocampus of the hypoxia and normal rats. Compared to the normal rats, the Fyn protein was significantly decreased at 6 weeks after hypoxia rats. In addition, the Fyn mRNA in the hippocampus of the hypoxia rats by RT-PCR were significantly decreased. Conclusion： These results suggest that Fyn may be involved in the mechanism of learning and memory resulting from hypoxia.

关 键 词：低氧 海马 FYN 大鼠 

分 类 号：R979.1[医药卫生—药品]