MicroPET观察姜黄素对APPswe/PS1dE9双转基因小鼠脑葡萄糖代谢的影响  

MicroPET observation of the effect of curcumin on cerebral glucose metabolism in APPswe/PS1dE9 double transgenic mice

作　　者：王虹[1] 高凯[2] 李瑞晟[1] 任映[1] 朱志慧[1] 孙海芸[1] 杨金铎[1] 王蓬文[1] 

机构地区：[1]北京中医药大学东直门医院中药药理学实验室,北京100700 [2]中国医学科学院医学实验动物研究所,北京100021

出　　处：《中国实验动物学报》2011年第4期336-338,I0011,共4页Acta Laboratorium Animalis Scientia Sinica

基　　金：北京市自然基金资助项目(No.7092057);国家自然基金资助项目(No.81073076)

摘　　要：目的利用18氟-脱氧葡萄糖microPET(18F-FDG microPET)影像学技术观察正常对照组小鼠,模型组和姜黄素治疗组APPswe/PS1dE9双转基因小鼠的脑葡萄糖代谢情况。方法随机挑选6月龄正常对照组C57BL/6J小鼠3只,模型组和姜黄素治疗组APPswe/PS1dE9双转基因小鼠各3只,通过2%异氟烷吸入麻醉后,从尾静脉弹丸式注射放射性示踪剂18F-FDG每例约14.8～16.5 MBq,摄取45 min后进行10 min microPET图像采集。计算并比较各组小鼠每克脑组织(除小脑)18F-FDG的摄取率。结果姜黄素治疗组小鼠每克脑组织18F-FDG的摄取率高于正常对照组和模型组。结论姜黄素能明显提高APPswe/PS1dE9双转基因小鼠脑18 F-FDG的摄取及每克脑组织的摄取率,并可能通过影响脑葡萄糖代谢而发挥神经保护作用。Objective To observe the status of cerebral glucose metabolism in normal control group mice,APP/PS1 double transgenic mice of model group and curcumin treatment group by 18F-FDG microPET.Methods APP/PS1 dtg mice from the model group,curcumin treatment group,and normal C57BL/6J mice with the same background were randomly selected（age： 6 months;n=3）for microPET study.Each mouse was anesthetized using 2% isoflurane and was injected through a tail vein with 14.8-16.5 MBq 18F-FDG as a bolus.After 45-minute of 18F-FDG uptake period,PET scans were performed for 10-min.Calculate and compare 18F-FDG uptake rate per gram tissue of the whole brain（except cerebellum） in the mice of different groups.Results Cerebral 18F-FDG uptake rate per gram tissue of curcumin treatment group mice is higher than both the normal control group and model group mice.Conclusions Curcumin can increase cerebral 18F-FDG uptake and 18F-FDG uptake rate per gram tissue.Curcumin may play a neuroprotective role through an influence to cerebral glucose metabolism.

关 键 词：18氟-脱氧葡萄糖 脑葡萄糖代谢 姜黄素 APPswe/PS1dE9双转基因小鼠 

分 类 号：Q95-33[生物学—动物学]