微针条件下秦艽复杂成分体系的透皮给药研究  

Transdermal delivery of Gentiana macrophylla complex components system under micro-needle conditions

作　　者：豆婧婧[1] 闫菁华[1] 徐坤[1] 陈贵[1] 惠先[1] 鞠大宏[2] 郝保华[1] 

机构地区：[1]西北大学生命科学学院,陕西西安710069 [2]中国中医科学院中医基础理论研究所,北京100700

出　　处：《药学学报》2011年第9期1137-1143,共7页Acta Pharmaceutica Sinica

基　　金：国家重大新药创制科技重大专项(2009ZX09502-019);陕西省中医药管理局科研基金(2007-25-05)

摘　　要：本文探讨了秦艽复杂成分体系在微针条件下透过不同部位皮肤的给药特点。采用双室扩散池,分别以离体小鼠不同部位皮肤和微针预处理离体小鼠不同部位的皮肤作为透皮屏障,用高效液相色谱(HPLC)相似度评价接收池中秦艽复杂成分体系的透皮特点及小鼠不同部位皮肤的龙胆苦苷的透过速率和透过量。被动给药和微针条件下给药24 h,接受池中秦艽复杂成分体系与原液相似度基本在83.0%～98.9%之间;微针预处理小鼠不同部位皮肤后,通过腹部皮肤给药接收液中秦艽复杂成分体系与原液相似度达到90%的时间为4 h;而背部皮肤和颈部皮肤给药时,则接收液中秦艽复杂成分体系与原液相似度达到90%的时间分别为18 h和12 h。微针能较理想的用于中药复杂成分的透皮给药,极大地缩短透皮吸收的时滞,提高其生物利用度;相对颈部和背部皮肤,微针通过腹部皮肤更能显著提高中药多成分透皮给药的速率及与原液的相似度。The purpose of this study is to investigate the transdermal delivery characteristics of Gentiana macrophylla complex components system through different parts of the skin under micro-needles conditions.Two-chamber diffusion cells were used,different parts of isolated skin and micro-needle pretreated isolated mouse skin were applied separately,high performance liquid chromatography（HPLC） similarity evaluation methods were used to evaluate transdermal delivery characteristics of Gentiana macrophylla complex components system on receiving pool and the permeation rate and penetration amount of Gentiopicroside at different parts of mouse skin.In the 24 h,the similarity between receiving fluid which was on passive transdermal delivery and micro-needle transdermal delivery conditions and original fluid were ranged from 83.0% to 98.9%;By the micro-needle pretreatment with different parts of the mouse skin,the time that Gentiana macrophylla complex components system though abdominal skin to the receiving fluid which reached 90% similarity compared with that of original fluid was 4 h,which was 18 h at back skin and 12 h at neck skin separately.Micro-needles can be used as the ideal ingredients for traditional Chinese medicine complex transdermal delivery;transdermal absorption time delay could be greatly reduced and its bioavailability was improved.The permeation rate and similarity to original liquid of Chinese medicine complex components increased significantly in the abdominal skin relative to the neck and back skin under micro-needle conditions.

关 键 词：秦艽 皮肤给药 高效液相色谱 色谱指纹图谱 

分 类 号：R943[医药卫生—药剂学]