铁皮石斛野生居群基于RAMP标记的遗传多样性评价  

Evaluation of genetic diversity of Dendrobium officinale wild populations based on RAMP markers

作　　者：沈洁[1,2] 徐慧君[1] 袁英惠[1] 韩丽[1] 侯北伟[1] 丁小余[1] 

机构地区：[1]南京师范大学生命科学学院,江苏南京210046 [2]连云港师范高等专科学校,江苏连云港222000

出　　处：《药学学报》2011年第9期1156-1160,共5页Acta Pharmaceutica Sinica

基　　金：国家自然科学基金资助项目(30870234);国家基础人才培养基金资助项目(J0730650);江苏省自然科学基金资助项目(BK2008431)

摘　　要：本文运用RAMP标记16对引物组合,对铁皮石斛9个野生居群的112个样本进行了检测,共得到123条扩增带,其中86条(占69.92%)具有多态性,每对引物组合可扩增出3～8条多态性带,平均5条,表明铁皮石斛不同居群间存在较丰富的遗传多样性;铁皮石斛居群间遗传相似系数的变化范围为0.250～0.813,平均值为0.550。利用RAMP扩增条带数据进行聚类分析,可将9个铁皮石斛野生居群划分为3个类群,聚类结果表现出较好的地域相关性,显示RAMP标记可以有效地评价铁皮石斛野生居群的遗传多样性及遗传结构。Random amplified microsatellite polymorphism（RAMP） markers were used to access the genetic diversity among 112 samples of nine populations of Dendrobium officinale Kimura et Migo.Using 16 informative primers,123 bands were amplified and 86（69.92%） were polymorphic.The polymorphic bands from three to eight could be detected for each RAMP primer,with a mean of 5,indicating abundant genetic diversity among populations.Genetic similarity coefficients ranged from 0.250 to 0.813.UPGMA dendrogram illustrated 9 populations clustered into 3 groups,and the cluster pattern showed correlation with the locations of the D.officinale populations.These results were supported by the previous conclusions that were achieved by other molecular markers,and RAMP is proved to be effective for evaluating the genetic diversity of wild populations of Dendrobium officinale.

关 键 词：铁皮石斛 居群 遗传多样性 RAMP 

分 类 号：R931[医药卫生—生药学]