法舒地尔对敌敌畏中毒大鼠急性肺损伤时血浆肿瘤坏死因子α和白细胞介素-6水平的影响  

Effect of Fasudil on Plasma Level of Tumor Necrotic Factor-α and Interleukin-6 on Acute Lung Injury in Rats with Dichlorvos Poisoning

作　　者：汤罗嘉[1] 姚晨玲[1] 黄培志[1] 

机构地区：[1]复旦大学附属中山医院急诊科,上海200032

出　　处：《中国临床医学》2011年第4期425-426,共2页Chinese Journal of Clinical Medicine

基　　金：国家科技支撑计划专题基金(编号:2006BAI06B01-)

摘　　要：目的:探讨法舒地尔(Fasudil)对敌敌畏中毒大鼠急性肺损伤时血浆肿瘤坏死因子α(TNF-α)和白细胞介素-6(IL-6)水平的影响。方法:Sprague Dawley大鼠30只,随机分成对照组、敌敌畏(dichlorvos,DDVP)中毒组、法舒地尔干预组3组,每组10只,均予以气管切开接呼吸机辅助通气。6h后留取血样用酶联免疫吸附分析(ELISA)法测血浆TNF-α、IL-6水平。取左肺下叶组织,HE染色观察病理学改变。结果:与对照组比,中毒组和干预组大鼠血浆TNF-α、IL-6水平均显著升高(P<0.01),肺组织HE染色有炎症细胞浸润;与中毒组比,干预组TNF-α、IL-6升高程度显著降低(P<0.05),肺损伤程度较轻。结论:法舒地尔能降低敌敌畏中毒大鼠血浆TNF-α、IL-6水平,能显著减轻肺损伤的病理损害,对大鼠敌敌畏中毒性急性肺损伤有明显的保护作用。Objective：To investigate the effect of fasudil on acute lung injury in rats with Dichlorvos poisoning.Methods： 30 male Spragne-Dawley（SD） rats were randomized into the control group,Dichlorvos poisoning group and Fasudil pretreatment group（n=10each）.Three groups were all supported with breathing machine connected with the tube from incision of trachea.tumor necrotic-α（TNF-α）,and interleukin-6（IL-6） in blood serum were measured six hours later.Histomorphology of lung tissue was observered though HE stain.Results： Compared with the control group,the level of TNF-α and IL-6 of the other two groups were higher（P0.01）.Compared with the poisoning group,the level of TNF-α and IL-6 were significant decreased in the pretreatment group（P0.05）.The histological changes of lung injury in the pretreatment group were weakened.Conclusions： It showed that Fasudil could inhibit the level of TNF-α and IL-6 in serum of rats with acute Dichlorvos poisoning.It could also decrease the histomorphology of lung injury.The protective effection on acute lung injury in rats with Dichlorvos poisoning was obviously.

关 键 词：敌敌畏 法舒地尔 肺损伤 肿瘤坏死因子Α 白细胞介素-6 

分 类 号：R595.4[医药卫生—内科学]