hTERC基因表达在宫颈病变筛查中的意义  

Evalution of gene expression of the human telomerase gene in the screating of cervical lesions

作　　者：周波[1] 李婷[1] 周晨[1] 陈忠东[1] 谢宛玉[1] 

机构地区：[1]南华大学第一附属医院妇产科,湖南衡阳421001

出　　处：《中国妇幼保健》2011年第26期4077-4080,共4页Maternal and Child Health Care of China

基　　金：卫生部科研基金资助项目〔WKJ2007-3-001〕

摘　　要：目的:探讨人端粒酶RNA(hTERC)基因在宫颈病变筛查中的临床意义。方法:收集2008年1月～11月南华大学第一附属医院115例妇女宫颈脱落细胞标本,病理学检查确诊CIN患者69例,宫颈鳞癌(SCC)患者26例,正常细胞学妇女20例。用荧光原位杂交(FISH)方法检测脱落细胞hTERC基因的表达。结果:①hTERC基因在CINⅠ、CINⅡ、CINⅢ及SCC患者宫颈脱落细胞中的阳性表达率分别是22.00%、75.00%、100.00%和100.00%。CINⅠ、CINⅡ、CINⅢ和SCC组与正常组比较,hTERC基因阳性率差异有统计学意义(P<0.05)。其中CINⅠ与CINⅡ、CINⅢ比较,CINⅠ与SCC比较差异有统计学意义(P<0.05)。②随着病变程度增加,hTERC基因的表达率增加。在CINⅠ中hTERC基因扩增的异常细胞仅占6.5%,而在CINⅡ、CINⅢ及SCC患者宫颈脱落细胞中,hTERC基因扩增的异常细胞所占比例依次递增为25.50%、30.60%和50.80%(P<0.05)。FISH检测结果显示,CINⅠ组细胞中hTERC基因异常扩增信号为2∶3型占60.00%,而在SCC中仅占36.44%。SCC中,4∶4型和5∶5型所占比例最多,其中5∶5型拷贝数是各级CIN的总和。hTERC基因的表达水平与宫颈病变的程度关系密切。结论:hTERC基因在CIN和SCC中表达异常,且随病变程度增加阳性表达率增加,可作为宫颈癌前病变进展的生物遗传学检测指标。Objective：To investigate the human telomerase RNA（hTERC） gene in the screating of cervical lesions. Methods：The expression of hTERC gene of cervix cytologic samples were detectded by using fluorescence in situ hybridization（FISH）.According to histology biopsy 115 Pap smears were divided into cervinl intraepithelial neoplasin（CIN,n=69）,squamous carcinomal of the cervix（SCC,n=26）.normnl（n=20）. Results：The positive rate of hTERC gene in CIN I,CIN II,CIN Ⅲ and SCC were respectively 22.00%,75.00%,100.00%and 100.00%（P〈0.05）.hTERC gene copy numbers in CINII/CINⅢ/SCC were significantly higher than that in CIN I.The number of abnormal cells were increased in gene amplification for hTERC,CIN I was 6.50%,CIN II was 25.50%,CINⅢ was 30.60%,SCC was 50.80%（P〈0.05）.The abnormal hTERC signal types of 2∶3 founded in CIN I was 60.00%,in CIN II,CINⅢ and SCC were 47.10%,45.18% and 36.44% respectively.In SCC,the abnormal hTERC signal types were 4∶4 and 5∶5 mainly. Conclusion：The increase of hTERC expression in the invasive CIN and SCC,the copy numbers are associated with the severity of cytologic and histologic findings.Therefore can as a screening detection index of biometrical genetics for progress of cervical anterior lesions.

关 键 词：荧光原位杂交 细胞遗传学 HTERC基因 宫颈上皮内瘤变 

分 类 号：R711.74[医药卫生—妇产科学]