高迁移率族蛋白B1在高氧致支气管肺发育不良中的表达  

Expression of high mobility group protein-B1 in bronchopulmonary dysplasia induced by hyperoxia exposure

作　　者：赵国英[1] 冯洁[1] 罗瑶[1] 

机构地区：[1]滨州医学院附属医院小儿内科,山东滨州256603

出　　处：《中国妇幼保健》2011年第26期4092-4095,共4页Maternal and Child Health Care of China

基　　金：国家自然科学基金项目〔30772036〕;山东省科技攻关项目〔2009GG20002055〕;山东省优秀中青年科学家科研奖励基金〔BS2010TT033〕

摘　　要：目的:检测高迁移率族群蛋白B1(HMGB1)在高氧(60%O2)暴露新生小鼠肺组织损伤模型中的表达水平,探讨HMGB1在支气管肺发育不良发病(Bronchopulmonary dysplasia,BPD)机制中的作用。方法:新生足月C57BL/6小鼠随机分为实验组和对照组,每组10只,制备60%氧浓度致新生鼠BPD模型,应用HE染色、Masson染色、放射性肺泡计数(RAC)、免疫荧光和实时荧光定量-PCR技术,观察生后3天、7天、14天肺组织病理改变,HMGB1的蛋白和mRNA表达水平。结果:氧处理组随时间推移,出现肺泡上皮肿胀,肺泡壁增厚,间质水肿,炎症细胞浸润,胶原样物质产生,肺泡结构紊乱,数量减少,较空白对照组明显发育迟滞。HMGB1蛋白和mRNA在3天氧处理组、空白对照组表达差异无统计学意义(P>0.05),7天、14天氧处理组表达均强于相应空白对照组(P<0.05)。结论:在60%浓度氧暴露所致BPD中,HMGB1表达增加;BPD的病理过程可能与HMGB1表达增加有关。Objective：To investigate the effect of high mobility group protein-B1（HMGB1） expression in newborn mouse with moderate hyperoxic exposure and the role of HMGB1 in the mechanism of bronchopulmonary dysplasia（BPD）.Methods：C57BL/6 mice were randomly divided into a BPD group and a control group.BPD model was established by exposure to 60% O2 in the neonatal period of C57BL/6 mice.Mice exposed to air were used as control groups,with 10 animals in each group on repeated experiments.The pathology of pulmonary tissue was detected by HE stain,Masson stain and radical alveolar counts（RAC）.The expression of HMGB1 protein in lung were detected by immunofluorescence and the expressions of HMGB1 mRNA by real-time fluorescent quantization PCR. Results：In BPD groups,lungs developed decreased alveolar septation,swelled alveolar epithelium,stroma edema,interstitial fibrosis and developmental lag compared with control groups.These changes became more obvious with more prolonged hyperoxia exposure.The expression of HMGB1 protein after 7 and 14 days of exposure increased significantly in the hyperoxia group compared with controls.The expression of HMGB1 mRNA was also higher after 7 and 14 days of exposure. Conclusion：Hyperoxia caused progressive addition in lung HMGB1 expression as well as abnormalitilies of lung structures,including decreased grouth and impaired alveolarization.These histologic changes are similar to those of BPD.The pathologic process of BPD may relate with the increased expression of HMGB1.

关 键 词：新生小鼠 高氧 支气管肺发育不良 高迁移率族蛋白B1 

分 类 号：R722[医药卫生—儿科]