西安地区血培养标本分离的MRSA mecA与qacA/B基因检测  

DETECTION ON mecA AND qacA/B GENE OF MRSA ISOLATED FROM XI'AN BLOOD CULTURE SAMPLES

作　　者：张志明[1] 武冬娜 徐永妮[3] 

机构地区：[1]西安市中心医院检验科,西安710003 [2]陕西省妇幼保健院 [3]解放军第323医院

出　　处：《中国消毒学杂志》2011年第5期556-558,共3页Chinese Journal of Disinfection

基　　金：陕西省卫生厅科学研究基金项目(H0813)

摘　　要：目的了解西安地区血培养标本中分离的耐甲氧西林金黄色葡萄球菌(MRSA)mecA和qacA/B基因携带情况,以便推测其抗药性。方法采用细菌培养鉴定技术和基因扩增方法,对收集2008年1月-2010年2月期间西安地区多家住院与门诊患者血培养标本进行了分离和检测。结果在2年内从西安地区3所医院血培养标本中分离鉴定出56株MRSA,mecA基因均为阳性,携带率为100%;有7株MRSA检出qacA/B基因阳性,携带率为12.5%。结论西安地区部分医院血培养标本分离的MRSA检测出qacA/B基因阳性率为12.5%,携带率较低。Objective To investigate mecA and qacA/B gene of methicillin - resistant Staphylococcus aureus isolated from blood culture samples from Xian in order to speculate the resistance. Methods Bacterial cuhure techniques and gent am- plification methods were used to separate and detect blood culture specimens including inpatient and outpatient in Xian hos- pital from January 2008 to February 2010. Results MecA gene was positive in all 56 strains MRSA isolated and identified within 2 years from three hospital in Xian blood cultures samples. 7 strains MRSA detection of qacA / B gene were posi- tive, and the positive rate was 12.5%. Conclusion QacA/B gene - positive rate of MRSA isolated from blood euhure samples in Xi^n hospital was low.

关 键 词：耐甲氧西林金黄色葡萄球菌 nlecA基因 qacA/B基因 抗药性 

分 类 号：R446.5[医药卫生—诊断学]