不同采样方法对腹腔镜器械灭菌监测结果的影响  

INFLUENCE OF DIFFERENT SAMPLING METHODS ON STERILIZATION RESULTS DETECTION OF LAPAROSCOPIC INSTRUMENTS

作　　者：杜合英[1] 王琼娟[1] 张晋昕[2] 刘明华[2] 李济宾[2] 罗春华[3] 

机构地区：[1]中山大学附属第一医院,广州510080 [2]中山大学公共卫生学院 [3]宁波市第一医院

出　　处：《中国消毒学杂志》2011年第5期590-591,共2页Chinese Journal of Disinfection

基　　金：广东省护理学会科研立项B2009031

摘　　要：目的比较不同采样方法对腹腔镜器械灭菌监测结果的影响。方法采用活菌计数检测方法,对4种采样法比较腹腔镜器械灭菌效果。结果腹腔镜分离钳轴关节和咬合齿单独采用涂擦法采样,阳性率为2.78%,而单独采用冲洗法采样,细菌培养全部为阴性结果;采用浸泡+反复冲洗法采样,细菌培养阳性率为16.67%。经统计学2х检验,两组涂擦法采样结果之间差异无统计学意义;冲洗法与浸泡+反复冲洗法的监测结果之间差异有统计学意义(P值<0.05)。结论采用浸泡+反复冲洗法采样,监测结果能比较客观,具有良好的可操作性。Objective To compare the influence of different sampling methods on sterilization results detection of laparo- scopic instruments. Methods Viable count detection method was used to compare sterilization results of laparoscopic in- strument among 4 sampling methods. Results Axle joint and bite tooth of laparoscopic separation clamp were sampled only by inunctions method and the positive rate was 2.78% , while bacterial culture results were negative by irrigation sampling method. Bacterial positive rate was 16.67% which sampled by soaking with repeatedly irrigation~ By test, simple results have no statistically significant differences between two different inunctions methods, test results between irrigation and soa- king with repeated irrigation were statistically significant differences （ P 〈 0.05 ）. Conclusions Sample by soaking with repeated .irrigation can reflect sterilization effects of instruments objectively and have ~ood operability.

关 键 词：灭菌 腹腔镜 灭菌效果 采样方法 

分 类 号：R187[医药卫生—流行病学]