香蕉枯萎病拮抗内生菌的分离、筛选及盆栽防效试验  

Isolation,screen and potted test of an endophytic bacterial against Fusarium oxysporum

作　　者：谢颖[1] 张孝峰 王瑀莹[1] 张俊威[1] 骆焱平[1] 

机构地区：[1]海南大学环境与植物保护学院,海南儋州571737 [2]鄂尔多斯市农业多种经营工作站

出　　处：《植物检疫》2011年第5期4-7,共4页Plant Quarantine

基　　金：海南大学热带作物种质资源保护与开发利用教育部重点实验室项目(2010hckled-08);海南大学2010年本科生创新科研课题(HDCX2010014)

摘　　要：采用组织表面消毒法分离变叶木、益智、阴香、槟榔、椰子和菠萝蜜6种植物的内生菌,获得91株内生菌,其中内生细菌45株,内生真菌38株,内生放线菌8株。以香蕉枯萎病菌(Fusarium oxysporum f.sp.cubense)为靶标菌,对分离的内生菌株进行皿内拮抗活性筛选,发现内生真菌、内生放线菌抑菌效果不理想,内生细菌YXG2-3拮抗效果最好,抑菌带达20mm,其次是BYG2-5、BYJ5-1、YXJ2-2。对4株内生细菌进行盆栽试验,发现菌株YXG2-3防效最好,达到63.8%,与其他处理达极显著水平。Endophytic bacteria were isolated from Codiaeum variegatum, Alpinia oxyphylla, Cinnamomum burmannii, Areca catechu, Cocos nucifera, Artocarpus heterophyllus through surface disinfection method. 91 endophytes were isolated, which included 38 endophytic fungi, 45 endophytic bacteria and 8 endophytic actinomycetes. Antagonistic activity tried against Fusarium oxysporum f. sp. cabense. The results showed that： the inhibitory effect is not satisfactory about endophytic fungi and endophytic actinomycetes, the antagonistic bacteria YXG2 -3 was the best, inhibition zone reached up to 20 ram; followed by BYG2 -5,BYJ5 -1 ,YXJ2 -2. the potted plant was test about 4 strains of endophytic bacteria, control effect of YXG2 -3 were 63.8%, which were highly signif- icant compared with other bacteria.

关 键 词：内生菌 分离 筛选 香蕉枯萎病原菌 

分 类 号：S476[农业科学—农业昆虫与害虫防治]