基于ITS序列的曼陀罗属植物的分类学研究  

Taxonomic study of Datura:inferences from ITS sequences of nuclear ribosomal DNA

作　　者：魏莎莎[1,2] 傅怡宁[1] 薛华杰[1] 印丽萍[1] 

机构地区：[1]上海出入境检验检疫局,上海200135 [2]上海大学生命科学学院

出　　处：《植物检疫》2011年第5期12-16,共5页Plant Quarantine

基　　金：国家质检总局科研项目(200810787);上海出入境检验检疫局科研项目(HK2011-28)

摘　　要：根据曼陀罗属核糖体基因转录间隔区(ITS)序列设计通用引物,得到33个不同来源的曼陀罗属植物的ITS序列,并以小天仙子(Hyoscyamus bohemicus)为外群,应用遗传距离与系统树分析法对曼陀罗属植物之间的分类进行了初步探讨。结果表明:在供试样品中,紫花曼陀罗(Datura tatula)和曼陀罗(Daturastramonium)的ITS序列完全相同;多刺曼陀罗(Datura ferox)、栎叶曼陀罗(Datura quercifolia)和曼陀罗(Datura stramonium)之间的亲缘关系很近;紫花曼陀曼(Datura tatula)、曼陀罗(Datura stramonium)、无刺曼陀罗(Datura inermis)和光滑曼陀罗(Datura laevis)4个种间不存在遗传距离;传统分类中曼陀罗属的Dutra亚属内除光曼陀罗(Datura leichhardtii)和异色曼陀罗(Datura discolor)在ITS序列表现为比较独立的2个种外,其他各种间的亲缘关系也较近;在ITS序列上湿地曼陀罗(Datura ceratocaula)与曼陀罗(Daturastramonium)没有差异。rDNA ITS （Internal Transcribed Spacer） sequences of 33 different resource species from Datura were amplified with primers deriving from ITS of Datura genus. In order to evaluate the relationships among species in- Datura, ITS sequences were used to analyze their sequence divergence and the phylogennetic tree was constructed in this paper. As the results of this study ： It was exactly the same in the ITS sequences of Datura tatula and Datura stramonium; the relationship between Datura ferox, Datura quercifolia and Datura stramonium was very close ; there was no genetic distance between Datura tatula, Datura stramonium and Datura inermis, Datura laevis in our study; relationships between species of the sectionDutra were relatively close; the exception of Datura leichhardtii and Datura discolor were comparatively isolated on the phylogenetic tree and with larger genetic distance with both section Stramonium and section Dutra; Datura ceratocaula and Datura stramonium shared the same ITS sequence.

关 键 词：曼陀罗属 ITS序列 亲缘关系 

分 类 号：Q949[生物学—植物学]