电针对抑郁症大鼠海马超微结构的影响  

Effect of Electroacupuncture on Neuron Ultrastructure in Hippocampus of Depression Rats

作　　者：符文彬[1] 马瑞[1] 刘健华[1] 孙健[1] 罗家星[2] 白艳甫 樊凌[2] 粟胜勇[2] 陈海滨[2] 苏临荣[2] 

机构地区：[1]广东省中医院针灸科,广东广州510120 [2]广州中医药大学第二临床学院,广东广州510120 [3]广州市中西医结合医院,广东广州510800

出　　处：《广州中医药大学学报》2011年第5期495-498,563,564,共6页Journal of Guangzhou University of Traditional Chinese Medicine

基　　金：国家自然基金项目资助(编号:30772828);广东省自然科学基金项目资助(编号:07004846);教育部高校博士点专项科研基金资助(编号:20094425110005)

摘　　要：【目的】观察针刺对抑郁症大鼠海马超微结构的影响。【方法】选用SD成年大鼠32只,随机将大鼠分为空白组、模型组、针刺组和西药组(盐酸氟西汀,剂量为1.8 mg.kg-1.d-1),每组8只。空白组进行正常饲养,不给予其他处理;模型组大鼠采用长期不可预见性温和刺激复制抑郁症模型;针刺组大鼠造模后针刺合谷穴和太冲穴,并加电针,每日1次,每次15 min,左右两侧穴位交替进行,持续21 d;西药组大鼠造模后给予氟西汀灌胃,每日1次,持续21 d。应激后22 d以脱臼法处死大鼠,取大鼠海马组织进行尼氏染色和透射电镜观察。【结果】模型组大鼠海马神经元数目减少,超微结构发生明显改变,神经元损害严重;针刺组和西药组大鼠海马神经元超微结构变化相对较轻,神经元受损程度明显轻于模型组。【结论】针刺可以逆转慢性应激所造成的抑郁大鼠海马神经元的损伤,这可能是针刺抗抑郁的物质基础和作用机制之一。Objective To explore the effect of electroacupuncture（EA） on the neuron ultrastructure in hippocampus of depression rats.Methods Thirty-two adult Sprague-Dawley rats were randomly divided into four groups： blank control group,model group,western medicine group and EA group（EA on points of Hegu and Taichong）,8 rats in each group.Rats in the blank control group did not receive any stimulation during the modeling,and rats in the model group were induced depression by chronic mild stress.After stress stimulation,rats in the western medicine group were treated with fluoxertine hydrochloride in the dosage of 1.8 mg·kg^-1·d^-1 for 21 days,and EA group was given EA stimulation for 15 minutes,once per day for 21 days.On the 22nd day of stress,rats were executed with neck-dislocating way,and the hippocampus was taken for Nissl’s staining and transmission electron microscopy（TEM）.Results The number of neurons was decreased,and significant ultrastructure changes and severe damage of neurons in the hippocampus were found in the model group.The ultrastructure changes and damage of neurons in the hippocampus of western medicine group and EA group were markedly mild as compared with those in the model group.Conclusion EA shows an effect on reversing the neural damage in the hippocampus of depression rats induced by chronic stress,which is one of the possible mechanisms of acupuncture against depression.

关 键 词：抑郁症/针灸疗法 海马/病理学 海马/超微结构 疾病模型 动物 大鼠 

分 类 号：R245.97[医药卫生—针灸推拿学]