甜菜M14品系花器官蛋白质双向电泳体系的建立  

Construction of two-dimensional electrophoresis technology platform of floral organ in sugar beet monosomic addition line M14

作　　者：李海英[1,2] 吴川[1,2] 曹洪祥[1,2] 杨乐[1,2] 马春泉[1,2] 于冰[1,2] 陈思学[1,2] 

机构地区：[1]黑龙江大学生命科学学院黑龙江省普通高等学校分子生物学重点实验室,哈尔滨150080 [2]黑龙江大学农业微生物技术教育部工程研究中心,哈尔滨150500

出　　处：《黑龙江大学自然科学学报》2011年第4期546-550,共5页Journal of Natural Science of Heilongjiang University

基　　金：国家自然科学基金资助项目(3087156631071473);黑龙江大学高层次人才(创新团队)支持计划(Hdtd2010-05)

摘　　要：双向电泳是目前唯一能将数千种蛋白同时分离与展示的技术。以具有野生白花甜菜优质基因资源的甜菜M14品系为材料,从植株取材、样品制备、蛋白质定量、上样量、双向电泳、染色、脱色等方面优化,构建了高效、稳定的甜菜M14品系花器官蛋白质双向电泳技术平台。结果证明所获得的凝胶图谱背景清晰,对比度高,重复性好,蛋白质点边缘平滑,并且低丰度的蛋白也能够很好的显现。The two-dimensional electrophoresis is the only technology that can simultaneously separate and display thousands of proteins. The floral organ of sugar beet monosomic addition line M14 was used as experiment material, which had high-quality genetic resources derived from Beta corolliflora Zoss. A highly efficient and stable two-dimensional electrophoresis technology platform of floral organ in M14 had been built by optimization in sample prepa- ration, protein quantification, the sample volume, two-dimensional electrophoresis, dyeing and bleaching, etc. The comparative experimental results showed that the 2-D gel map had the features of clear background, high contrast, good reproducibility, smooth edges of proteins. Meanwhile, low abundance proteins also could be a good show.

关 键 词：甜菜M14品系 蛋白质组学 双向电泳 

分 类 号：S566.3[农业科学—作物学]