重组天花粉蛋白的真核表达及其对人宫颈癌Caski细胞增殖的影响  

Effect of recombinant trichosanthin on proliferation of human cevical cancer Caski cells

作　　者：彭平平[1] 黄利鸣[1] 王艳林[2] 尤程程[1] 曹卫红[1] 宋华梅[2] 谭寒星[2] 吴燕珍[2] 

机构地区：[1]三峡大学第二临床医学院,湖北宜昌443002 [2]三峡大学分子生物学研究所,湖北宜昌443002

出　　处：《中国中药杂志》2011年第18期2539-2542,共4页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(30873282);湖北省自然科学基金项目(2009CDA060)

摘　　要：目的:观察重组天花粉蛋白(recombinant trichosanthin,rTCS)高表达对人宫颈癌Caski细胞增殖的影响。方法:构建真核表达质粒pcDNA3.1(-)/6His-TCS,稳定转染Caski细胞后,RT-PCR,Western-blot筛选rTCS高表达细胞株。以转染空载质粒pcDNA3.1(-)细胞株作为对照组,MTT法检测2组细胞的增殖情况,流式细胞术检测细胞周期变化。结果:成功获得高表达rTCS的Caski细胞株,rTCS高表达能够显著抑制Caski细胞增殖(P<0.01),并能将细胞抑制在G1和G2期(P<0.05)。结论:rTCS在细胞内高表达具有抗人宫颈癌细胞生长的作用,该研究为宫颈癌治疗提供了新的思路。Objective： To observe the effects of high expression of recombinant trichosanthin（rTCS） on the cell proliferation and cell cycle of human cervical cancer Caski cells.Method： Eukaryotic expression plasmid pcDNA3.1（-）/6His-TCS was constracted and stably transfected into Caski cells.RT-PCR,Western-blot were used to select the clones with rTCS high-expressing.Using pcDNA3.1（-）-transfected cells as the control,MTT assay and flowcytometry were used to elucidate the effects of rTCS high expression on cell growth and cycle regulation in Caski cells.Result： The Caski cells with stable high expression of rTCS was successfully established,which could inhibit the cell growth（P〈0.01） and arrest Caski cells in G1 and G2 phases（P〈0.05） obviously.Conclusion： High expression of rTCS can inhibit the growth of Caski cervical cancer cells,which might provide a new pathway for the therapy of cervical cancer.

关 键 词：重组天花粉蛋白 宫颈癌 细胞增殖 

分 类 号：R285[医药卫生—中药学]