采用化学药物联合致小鼠血虚模型评价四物汤及其配伍组成的作用特点  

Studies on influence of Siwu decoction and its composite drugs on chemical-induced blood-deficiency model mice

作　　者：朱敏[1] 段金廒[1] 唐于平[1] 宿树兰[1] 华永庆[1] 郭建明[1] 钱大玮[1] 

机构地区：[1]南京中医药大学江苏省方剂研究重点实验室,江苏南京210046

出　　处：《中国中药杂志》2011年第18期2543-2547,共5页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(30873235);国家科技支撑计划项目(2008BAI51B01);江苏省自然科学基金项目(BK2008455);江苏省高校自然科学重大基础研究项目(06KJA36022;07KJA36024;10KJA360039);国家教育部"新世纪优秀人才支持计划"(NCET-09-0163);江苏省方剂高技术研究重点实验室建设项目(BM2010576);江苏省理血方剂创新药物工程中心建设项目

摘　　要：目的:研究四物汤组方对乙酰苯肼及环磷酰胺致血虚模型小鼠的影响,探讨其组方特点和作用机制。方法:用乙酰苯肼和环磷酰胺造小鼠血虚模型,分别采用全自动血细胞分析仪测定血虚小鼠外周血象,称重法测定肝脾指数,定磷法测定红细胞膜Na+-K+-ATP酶,Ca2+-Mg2+-ATP酶活性,流式细胞仪检测骨髓细胞周期。结果:其中药味当归、白芍对模型小鼠的外周血象、肝脾指数、骨髓细胞周期均有较显著的恢复作用,熟地对红细胞膜Na+-K+-ATP酶活性的影响也较显著;含当归的药对对模型小鼠的肝脾指数、红细胞膜Na+-K+-ATP酶活性的影响则较显著;含白芍的药组和含当归的药组则分别对肝指数和红细胞膜Na+-K+-ATP酶活性有较显著影响;各给药组均能促进G0/G1期阻滞的骨髓细胞向S期转化。结论:四物汤及其组方药对化学损伤血虚模型小鼠均有不同程度的保护作用,作用途径各有特点,可见当归和白芍对血虚症状的改善有重要的作用,是四物汤发挥理血调经功效的重要贡献药味。Objective： To investigate Siwu decoction and its composite drugs on the blood-deficiency model mice induced by acetylphenyhydrazine and cyclophosphamide.Method： Acetylphenyhydrazine and cyclophosphamide were used to copy the blood-deficiency model mice.Automatic hematology analyzer was used to test the peripheral hemogram.Weighting method was used to test the liver index and spleen index;Kits for ATPase test was used to test the activities of Na＋-K＋-ATPase/Ca2＋-Mg2＋-ATPase in erythrocyte membrane.Flow cytometry was used to test the bone marrow cells′ cell cycle.Result： Angelicar Sinensis Radix and Paeoniae Radix Alba had the most effective activity on the peripheral hemogram.Paeoniae Radix Alba,the drug pair including Angelicar Sinensis Radix and the drug-group including Paeoniae Radix Alba had the most effective activity on the liver index.All the drugs,drug-pairs,drug-groups and the formula had effect on the spleen index.To the activity of Na＋-K＋-ATPase in erythrocyte membrane,Rehmanniae Radix Praeparata,the drug-pairs and drug-groups including Angelicar Sinensis Radix exhibited the most effective activity.All the drugs,drug-pairs,drug-groups and the formula had the protective effect on the damaged bone marrow cells.Conclusion： Siwu decoction and its composite drugs all had effect on the blood-deficiency model mice,but the action intensity was different.Angelicar Sinensis Radix and Paeoniae Radix Alba exhibited the most effective activity to the protection of the blood-deficiency model mice.

关 键 词：四物汤 血虚模型 配伍组成 作用特点 

分 类 号：R285.5[医药卫生—中药学]