滋补肝肾方含药血清对小鼠黑素瘤细胞酪氨酸酶活性及表达的调节作用  

Effect of zibuganshenfang on the expression and activity of tyrosinase on B16 cells

作　　者：赵京霞[1] 张蕾[1] 李萍[1] 刘欣[1] 王燕[1] 

机构地区：[1]首都医科大学附属北京中医医院北京市中医研究所,北京100010

出　　处：《中国中药杂志》2011年第18期2567-2570,共4页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(30572396);北京市自然科学基金项目(7062011)

摘　　要：目的:观察临床治疗白癜风的有效方剂滋补肝肾方含药血清对B16黑素瘤细胞酪氨酸酶活性及表达的影响,探讨其治疗白癜风的作用机制。方法:酶学方法研究滋补肝肾方含药血清对B16黑素瘤细胞酪氨酸酶活性的影响;RT-PCR和Western blotting技术检测其对TYR mRNA和蛋白表达的影响。结果:与相应浓度的空白血清对照组相比,各个浓度的滋补肝肾方含药血清对B16细胞的增殖无明显的促进作用。不同浓度滋补肝肾方含药血清与相应空白血清相比,可显著提高B16黑素细胞酪氨酸酶活性,其中20%含药血清与空白血清相比对黑素细胞酪氨酸酶活性促进作用最显著(P<0.01)。20%滋补肝肾方含药血清作用于B16细胞72 h,与20%空白血清相比,其Tyr mRNA,Trp-1 mRNA表达均显著升高(P<0.01),Tyr蛋白表达也升高。结论:滋补肝肾方含药血清对黑素细胞酪氨酸酶活性有正向的调节作用,并可促进Trp-1 mRNA,Tyr mRNA及蛋白的表达,这可能是滋补肝肾方临床治疗白癜风的机理之一。Objective： ZiBuGanShenFang（ZBGSF）is a traditional herbal formula,which has showed an outstanding therapeutic effect on vitiligo clinically.Our aim is to investigate the influence of ZBGSF drug serum on the expression and activity of Tyrosinase in B16 cells,explore the mechanism of ZBGSF on Vitiligo treatment further.Method： tyrosinase activity was measured by zymological methods,western blotting and RT-PCR were used to measure the protein content and mRNA level of tyrosinase and related proteins,respectively.Result： ZBGSF drug serum had no effect on the proliferation of B16 cells.But it could promote Tyrosinase activity significantly and increase protein content and mRNA level of tyrosinase and related proteins in B16 cells.Conclusion： Promoting the expression of tyrosinase protein and mRNA may be the elementary basis of ZBGSF on Vitiligo treatment.

关 键 词：酪氨酸酶 白癜风 中医药 

分 类 号：R285[医药卫生—中药学]