荧光染色发光二极管显微镜检测结核分枝杆菌的效果分析  

Efficiency of fluorescence microscopy with light emitting diodes in detecting Mycobacterium tuberculosis

作　　者：陈燕梅[1] 钱明[1] 江勇[1] 陈涛[1] 李海成[1] 钟球[1] 

机构地区：[1]广东省结核病防治研究所参比实验室,广州510630

出　　处：《中国防痨杂志》2011年第9期585-587,共3页Chinese Journal of Antituberculosis

基　　金：广东省医学科研课题项目(编号:A2009115);"艾滋病和病毒性肝炎等重大传染病防治"科技重大专项(2008ZX10003-007-02)

摘　　要：目的探讨荧光染色发光二极管(LED)显微镜镜检对结核分枝杆菌的检出效果。方法收集232例可疑结核病患者的痰标本,每位患者收集3份痰(即时痰、清晨痰和夜间痰),共696份痰标本。分为萋-尼染色(Z-N)法镜检组(简称Z-N组),荧光染色发光二极管显微镜镜检组(简称LED组)。对每份痰标本同时采用上述两种镜检法进行检测。比较两者的检查结果,分析两种方法检查结核分枝杆菌的阳性率差异情况。结果 LED组检出率为37.9%(88/232),Z-N组检出率为26.7%(62/232),经检验,χ2=6.66,P<0.01,差异有统计学意义。结论LED显微镜镜检具有阳性检出率高等优点。Objective To evaluate the efficiency of fluorescence microscopy with light emitting diodes（LED-FM） in screening Mycobacterium tuberculosis. Methods Sputum samples of 232 suspected patients were collected and there were totally 696 samples including 3 samples from each patient.All samples were detected by Ziehl-Neelsen（Z-N） stain microscopy and LED-FM respectively. Results The positive rates of LED-FM and Z-N were 37.9%（88/232） and 26.7%（62/232） with significant difference（χ^2=6.66,P〈0.01）. Conclusion LED-FM in detecting Mycobacterium tuberculosis is superior to the conventional method.

关 键 词：分枝杆菌 结核 显微镜检查 荧光 染色与标记 

分 类 号：R446.5[医药卫生—诊断学]