干扰素体外释放酶联免疫法在结核病诊断中的价值  

Diagnostic value of quantitative diagnostic kit for Mycobacterium tuberculosis-IFN-γ release assay

作　　者：刘佳文[1] 康丽军[1] 翁绳凤[1] 朱新颖[1] 

机构地区：[1]北京老年医院检验科,100095

出　　处：《中国防痨杂志》2011年第9期600-603,共4页Chinese Journal of Antituberculosis

基　　金："十一五"国家重大科技专项(2008ZX-10003-004)

摘　　要：目的分析干扰素体外释放酶联免疫法(quantitative diagnostic kit for Mycobacterium tuberculosisIFN-γrelease assay,TB-IGRA)在结核病诊断中的应用价值。方法用北京万泰生物药业股份有限公司生产的结核分枝杆菌相关γ-干扰素检测试剂盒(体外释放酶联免疫法)(Wantai quantitative diagnostic kit for Mycobacteriumtuberculosis IFN-γrelease assay,WT-IGRA)(简称WT试剂)和澳大利亚Cellestis有限公司生产的QuantiFER-ON-TB(QuantiFERON-TB GOLD in tube,QFT-GIT)(简称QFT试剂)检测病区与门诊结核患者(249例)及健康者(140例)的全血标本,结果与其结核菌素试验(PPD)、痰结核分枝杆菌培养及痰涂片结果相比较,分析TB-IGRA阳性率、特异度及试剂间的相关性。结果 249例结核病患者,WT试剂和QFT试剂阳性率分别为76.3%(190/249)和67.1%(167/249),特异度都是83.3%(100/120);痰菌阳性和痰菌阴性结核病组,WT试剂阳性率分别为74.1%(43/58)和76.1%(83/109),差异无统计学意义(χ2=0.082,P>0.05);QFT试剂阳性率分别为67.2%(39/58)和65.1%(71/109),差异无统计学意义(χ2=0.075,P>0.05);WT试剂与QFT试剂总符合率为84.3%。结论 TB-IGRA对结核病有一定的诊断价值。Objective To analyze the clinical value of Mycobacterium tuberculosis IFN-γ release assay（TB-IGRA） in diagnosis of tuberculosis. Methods The serum specimens of 249 patients with tuberculosis and 140 healthy people were detected by Mycobacterium tuberculosis-IFN-γ release assay with Wantai quantitative diagnostic kit（WT-IGRA,Beijing Wantai Biological Pharmacy Corporation Ltd.） and QuantiFERON-TB GOLD in tube（QFT-GIT,Cellestis Limited,Australia）,respectively.The results of TB-IGRA with WT-IGRA and QFT-GIT were compared with BCG-PPD skin test,Sputum smear and mycobacterial culture. Results Among 249 patients with tuberculosis,the positive rate of TB-IGRA with WT-IGRA and QFT-GIT were 76.3%（190/249）and 67.1%（167/249）,respectively,and the specificity with two methods were all 83.3%（100/120）.There were no statistical significant differences on positive rates of TB-IGRA and QFT-GIT between smear-positive patients and smear-negative patients which were 74.1%（43/58）,76.1%（83/109）with WT-IGRA（χ^2=0.082,P〉0.05） and 67.2%（39/58）,65.1%（71/109）with QFT-GIT（χ^2=0.075,P〉0.05）.The coincidence rate of the two kits is 84.3%. Conclusion TB-IGRA has certain diagnostic value in tuberculosis.

关 键 词：结核/诊断 干扰素Ⅱ型 酶联免疫吸附测定 

分 类 号：R52[医药卫生—内科学]