PI3K/Akt通路在滋养细胞增殖中的调控机制研究  

The regulation mechanism of PI3K/Akt pathway in trophoblast proliferation

作　　者：赵茵[1] 夏曙[2] 钟少平[1] 程红[1] 邹丽[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院妇产科,武汉430022 [2]华中科技大学同济医学院附属同济医院肿瘤科,武汉430030

出　　处：《中国优生与遗传杂志》2011年第10期72-74,83,共4页Chinese Journal of Birth Health & Heredity

基　　金：教育部博士点新教师基金资助编号:20070487174 20070487160

摘　　要：旨在探讨PI3K/Akt信号转导通路在滋养细胞增殖中的作用及具体调控机制。体外培养滋养细胞系EVT。应用MTT法检测不同浓度表皮生长因子(EGF)刺激后EVT的增殖情况;应用流式细胞技术检测不同处理组EVT凋亡情况。使用PI3K抑制剂LY294002处理细胞后,检测以上各项结果的变化。结果发现:1.随EGF浓度增高,EVT增殖呈现增强趋势,EGF在10ng/ml及其以上时效应明显;2.EGF能够显著降低EVT的凋亡发生;3.使用PI3K抑制剂LY294002明显逆转EGF的促进EVT增殖的效应。提示表皮生长因子可以活化滋养细胞的PI3K/Akt信号通路,进而促进细胞增殖,并且抑制其凋亡,PI3K抑制剂可以明显抑制EGF的促EVT增殖作用。To examine the possible involvement of the phosphatidylinositol 3-kinase（PI3K） pathway in extravillous trophoblast（EVT） proliferation regulated by EGF.EVT cell line（TEV-1） cells were cultured in free serum with EGF.PI3K inhibitor LY294002 was used to interfere with PI3K /Akt signalling pathway.The proliferation and apoptosis of TEV-1 cells were measured by MTT and flow cytometry assay.With the EGF concentration increasing,MTT reduction shows upward tendency.MTT reduction was inhibited by LY294002 significantly.Furthermore,EGF showed the decreasing effect on the apoptosis rate.PI3K antagonist LY294002 neutralized the improving proliferation effect of EGF.The results showed that PI3K /Akt pathway may be involved in the proliferation of EVT cells regulated by EGF.

关 键 词：PI3K/AKT 表皮生长因子 绒毛外滋养细胞 

分 类 号：R714.56[医药卫生—妇产科学]