大鼠PAG内Nogo-A在痛觉调制过程中角色的探索  

ROLE OF NOGO-A IN PAIN MODULATION IN PAG OF RAT

作　　者：孟蒂[1] 刘丹[1] 房春燕[1] 王燕[1] 李宁[1] 

机构地区：[1]潍坊医学院应用药理学实验室,山东潍坊261053

出　　处：《中国疼痛医学杂志》2011年第9期554-557,562,共5页Chinese Journal of Pain Medicine

基　　金：山东省自然科学基金资助(NO.ZR2009CL049)

摘　　要：目的:用脑内核团微量注射和免疫组化等方法,观察大鼠中脑导水管周围灰质(periaqueduc-tal gray,PAG)内"勿动蛋白"-A(Nogo-A)、P物质(substance P,SP)和降钙素基因相关肽(calcitoningene-related peptide,CGRP)表达的变化,研究探讨Nogo-A在痛觉调制过程中的角色,以及它在大鼠PAG内与CGRP、SP和内源性阿片系统在痛觉调制过程中的相互作用。方法:选用雄性SD大鼠32只,随机等分为4组:空白对照组8只,分别向PAG内注射0.9%生理盐水1μl;模型对照组、吗啡组和纳洛酮组各8只,首先单侧后爪掌心皮下注射1%的福尔马林0.1 ml复制炎症痛敏大鼠模型,3 d后分别向PAG内注射等量0.9%生理盐水、吗啡或纳洛酮各1μl,60 min后取大鼠大脑PAG区脑组织,采用免疫组化法观测各组大鼠PAG内Nogo-A、SP和CGRP免疫反应阳性神经元的表达差异。结果:与空白对照组比较,模型对照组大鼠PAG内Nogo-A的表达量显著降低(P<0.01),CGRP及SP显著增高(P<0.01);与模型对照组比较,吗啡组大鼠PAG内Nogo-A的表达量显著增高(P<0.01),CGRP及SP显著降低(P<0.05),而纳洛酮组PAG内Nogo-A的表达量显著降低(P<0.05),CGRP及SP显著增高(P<0.05)。结论:(1)炎症痛敏大鼠PAG内Nogo-A的表达量比正常降低,而CGRP及SP显著增高;(2)阿片肽可增加PAG内Nogo-A的表达,并降低CGRP及SP的表达。Objective：The present study was performed to observe the expression of Nogo-A,substance P（SP） and calcitonin gene-related peptide（CGRP） in periaqueductal gray（PAG） of hyperalgesia rats, and to investigate the role of Nogo-A in pain modulation,and the relationship with CGRP,SP and endogenous opioid system.Methods：32 male SD rats were randomly divided into four groups：control group received intra-PAG injection of 0.9%saline（1μl）;Model group,morphine group and naloxone group received unilateral hindpaw hypodermic injection of 1%formalin（0.1 ml） to prepare pain model,then received intra-PAG injection of 0.9%saline 1μl,morphine 1μl or naloxone 1μl respectively 3 days later.After 60 minutes,the PAG area of rats were obtained,the expression of Nogo-A,SP and CGRP in PAG of rats in each group were observed and measured by immunohistochemical method.Results：The expression of Nogo-A immunoreactive neuron of model group was obviously reduced than that of control group（P0.01）,but CGRP and SP was obviously increased（P0.01）;The expression of Nogo-A immunoreactive neuron of morphine group was obviously increased than compared with model group（P0.01）, but CGRP and SP was obviously reduced（P0.05）;The expression of Nogo-A immunoreactive neuron in naloxone group was obviously reduced than that in model group（P0.05）,but CGRP and SP was obviously increased（P0.05）.Conclusion：（1） The expression of Nogo-A immunoreactive neuron of model group was obviously reduced than that of control group,but the CGRP and SP were obviously increased. （2） The expression of Nogo-A immunoreactive neuron was obviously increased by opioid system in PAG,but the CGRP and SP were reduced.

关 键 词：中脑导水管周围灰质 降钙素基因相关肽 NOGO蛋白 P物质 痛觉调制 

分 类 号：R338[医药卫生—人体生理学]