HPLC法测定补肾生精丸中芍药苷的含量  

HPLC Determination of Paeoniforin in Bushenshengjing Pill

作　　者：丁广军[1] 

机构地区：[1]河南省商丘市第一人民医院,商丘476100

出　　处：《中国中医药现代远程教育》2011年第13期144-144,共1页Chinese Medicine Modern Distance Education of China

摘　　要：目的建立HPLC法测定补肾生精丸中芍药苷含量的方法。方法采用EclipseXDB-C18色谱柱,乙腈:0.1%磷酸溶液(14:86)为流动相;流速1.0ml·min-1;检测波长230nm。结果芍药苷在0.1332～1.1988μg范围内线性关系良好(r=0.9994),平均回收率为98.26%,RSD=1.29%(n=6)。结论本方法操作简便,专属性强,结果准确,可用于补肾生精丸的质量控制。Objective To establish a HPLC method to determination of paeoniforin in bushenshengjing pill.Method The chromatographic system consisted of C18 column,using acetonitrile-0.1% phosphate solution（14：86） as mobile phase.The contentwas detected at a waveleng of 230 nm,the flow rate was 1.0ml？min-1.Results The paeoniforin have a good tincar rolatim in the range of 0.1332～1.1988μg（r=0.9994）,the average recovery was 98.26% and RSD=1.29%（n=6）.Conolusion The established method is accuracy,sensitive and simple.It can be used for quality control of bushenshengjing pill.

关 键 词：芍药苷 高效液相色谱法 含量测定 

分 类 号：R286[医药卫生—中药学]