4味中药乙醇提取物祛黄褐斑作用的比较研究  

Effect of ethanol extracts from 4 Chinese crude drugs on chloasma

作　　者：毛骁丽[1] 陈光宇[1] 何群[2] 赖彬[3] 黄迎玉[3] 杨志波[3] 

机构地区：[1]湖南中医药大学中药乙醇提取物现代化实验室,长沙410208 [2]湖南中医药大学中药乙醇提取物药剂学重点学科,长沙410208 [3]湖南中医药大学第二临床医院皮肤科,长沙410005

出　　处：《中南药学》2011年第8期604-607,共4页Central South Pharmacy

摘　　要：目的比较白附子、银花、白芨、六月雪4味中药乙醇提取物祛黄褐斑作用的差异,选出作用最强的药材。方法以MTT法测定小鼠B16黑素瘤细胞增殖抑制率、酪氨酸酶活性、黑素含量变化;采用多重比较法进行统计分析。结果银花的浓度为62.5μg.mL-1,白芨、白附子各浓度(500、250、125、62.5μg.mL-1)均对B16鼠黑素瘤细胞增殖抑制作用较强。白附子的浓度为250μg.mL-1或银花的浓度为62.5μg.mL-1对小鼠B16黑素瘤细胞酪氨酸酶活性抑制作用最佳。白附子的浓度为125μg.mL-1对黑素合成抑制作用最好。结论白芨与白附子可作为祛黄褐斑有效成分的候选药材。Objective To distinguish the different effects of ethanol extracts from 4 Chinese crude drugs on chloasma,including Rhizome typhonii,Flos lonicerae,Rhizoma bletillae and Serissa serissoides.Methods Inhibition ratio of the cell proliferation of melanoma and tyrosinase activity in cultured B16 melanoma cells were measured by MTT method and multiple comparison method was employed in statistical analysis.Results Flos lonicerae at 62.5 μg·mL^-1,Rhizoma bletillae and Rhizome typhonii at various concentrations（500,250,125,and 62.5 μg·mL^-1）inhibited the proliferation of cultured B16 melanoma cells.Rhizome typhonii at 250 μg·mL^-1 or Flos lonicerae at 62.5 μg·mL^-1 inhibited cultured B16 melanoma cells and tyrosinase activity most;Rhizome typhonii at 125 μg·mL^-1 inhibited melanogenesis best.Conclusion Rhizoma bletillae and Rhizome typhonii may be considered to be candidates for active ingredients to remove chloasma.

关 键 词：黄褐斑 黑素瘤 细胞增殖 酪氨酸酶 抑制率 白附子 银花 白芨 六月雪 

分 类 号：R961[医药卫生—药理学]