核因子-κB活化在胃癌耐药细胞株中作用的实验研究  

Study on NF-κB activity in chemo-resistance to adriamycin in human stomach cancer cell line

作　　者：傅庭焕[1] 李基业[1] 裴利娟[1] 

机构地区：[1]解放军总医院第一附属医院普通外科,北京100037

出　　处：《中国急救复苏与灾害医学杂志》2011年第9期771-774,共4页China Journal of Emergency Resuscitation and Disaster Medicine

摘　　要：目的探讨核因子-κB活化在胃癌细胞株耐药性机制中的作用。方法诱导培养胃癌细胞耐药亚株SGC7901／ADM，MTT法检测阿霉素对胃癌细胞株的细胞毒作用，流式细胞仪检测胃癌细胞株凋亡率的变化，免疫细胞化学染色法检测胃癌细胞株中的核因子-κB活化。结果SGC7901／ADM相对耐药度较亲本细胞提高了8．9倍，10ug／ml阿霉素所导致的SGC7901／ADM胃癌细胞株凋亡率在48h最低达到17．53％＋1．02％，较亲本细胞下降31％。核因子-κB活化在SGC7901／ADM胃癌细胞株达到65％。结论核因子-κB活化所导致的胃癌细胞株凋亡率下降在SGC7901／ADM胃癌细胞株耐药性机制中发挥一定作用。Objective To study the function of inducible nuclear factor-kappaB （NF-κB） in chemo-resistance to adriamycin in human stomach cancer cell line. Methods A human stomach cancer cell line resistant to adriamycin （SGC7901/ADM） was acquired by stepwise-increasing concentrations of adriamycin in vitro. The cytotoxicity of ADM on SGC7901/ADM and SGC7901 stomach cancer cell line was determined by MTr assay. The level of NF-K B activity was measured by immunohistochemical staining. Results Relative degree of resistance to adriamycin in SGC7901/ADM was improved 8.9 times than that in parental cells. The apoptosis rate of SGC7901/ADM induced by lOug/ml adriamycin was 31% lower than SGC7901 stomach cell line. The level of activation of NF-κB in SGC7901/ADM reached 65%, which was significantly higher than SGC7901stomach cell line. Conclusion The change of apoptosis rate induced by NF-κB activity maybe play an important role in chemo-resistance.

关 键 词：核因子-ΚB 耐药性 胃癌细胞株 凋亡 

分 类 号：R45[医药卫生—治疗学]