聚酚藏花红功能碳纳米管生物阳极制备及其在乙醇传感器应用(英文)  

Development of Dehydrogenase-Based Bioanode Using Poly(Phenosafranin)-Functionalized SWCNT Nanocomposites and its Application to Ethanol Biosensor

作　　者：Farhana S.Saleh Takeyoshi Okajima 毛兰群[2] 大坂武男[1] 

机构地区：[1]东京工业大学电子化学系,日本东京226-8502 [2]北京分子科学国家实验室,中国科学院活体分析化学重点实验室,北京100190

出　　处：《电化学》2011年第3期263-270,共8页Journal of Electrochemistry

基　　金：supported by Grant-in-Aid for Scientific Research(A)(No.19206079)from the Ministry of Education,Culture,Sports,Science and Technology(MEXT),Japan,Tokyo Institute of Technology Global COE Program for Energy Science;Japan-China Research Program on Enzyme-based Biofuel Cells organized;sponsored by Japan Science and Technology(JST)and Natural Science Foundation of China(NSFC)

摘　　要：应用电化学法聚合酚藏花红(PPS)功能化的单壁碳纳米管,以其作为烟酰胺辅酶(NADH)氧化的电化学催化剂(电极),构建基于乙醇脱氢酶的安培型乙醇生物电化学传感器.该电极于0.0 V时,对NADH具有很好的催化性能.而单体酚藏花红则由于其电位过低(-0.48 V),不能显示催化性能.循环伏安和计时安培法测试表明:该传感器的碳纳米管的载量,固定化酶的量,NAD+的浓度以及溶液的pH都能直接影响它的性能.经优化制备的乙醇传感器在0.2 V电位下,对乙醇响应的灵敏度为2.0μA.cm-2.mM-1,检测限为36μmol.L-1.表现出很好的稳定性,连续测定45 min后,响应电流下降仅为起始值的7%.本文的研究为电化学生物传感器的创新开发提供了新的思路.A New type of dehydrogenase-based amperometric ethanol biosensor was constructed using alcohol dehydrogenase（ADH） which was immobilized on the edge-plane pyrolytic graphite（EPPG） electrode modified with poly（phenosafranin）-functionalized single-walled carbon nanotube（PPS-SWCNT）.The PPS-SWCNT modified EPPG electrode was prepared by electropolymerization of phenosafranin on the EPPG electrode which was previously coated with SWCNT.The performance of the ADH/PPS-SWCNT/EPPG electrode was evaluated using cyclic voltammetry and amperometry in the presence of ethanol.The fabricated ethanol biosensor provided a reasonable sensitivity of 2.0 μA·cm-2·mmol-1·L and a low detection limit（36 μmol·L-1） for the electrocatalytic oxidation of ethanol with a linear concentration dependence upto^1.0 mmol·L-1 at a detection potential of 0.2 V.

关 键 词：酚藏花红(PS) 电化学聚合 烟酰胺辅酶(NADH) 单壁碳纳米管(SWCNT) 电催化 

分 类 号：TP212.2[自动化与计算机技术—检测技术与自动化装置]