柠檬酸修饰(CA/GC)电极对多巴胺(DA)和肾上腺素(EP)同时检测  

Simultaneous Determination of Dopamine(DA)and Epinephrine(EP) at CA/GC Electrode

作　　者：任旺[1] 张英[1] 李敏娇[1] 

机构地区：[1]四川理工学院化学与制药工程学院,四川自贡643000

出　　处：《电化学》2011年第3期343-346,共4页Journal of Electrochemistry

基　　金：四川省教育厅基金项目(10ZB102);四川理工学院科技项目(2009xjky1001)资助

摘　　要：应用电沉积法制备柠檬酸修饰玻碳电极(CA/GC),差分脉冲法研究多巴胺(DA)和肾上腺素(EP)在该修饰电极上的电化学行为.结果表明,样品DA、EP在该电极的还原峰电位差380 mV,而抗坏血酸(AA)在此电位区无还原峰,因此可实现该修饰电极对DA和EP的同时检测,而且高浓度AA不发生干扰.在pH 6.0的磷酸盐缓冲液中,DA和EP还原峰电流与其浓度分别在1.0×10-6～6.0×10-5 mol.L-1和2.0×10-6～6.0×10-5 mol.L-1范围内呈线性关系.CA/GC电极制备简单,重现性好,可望用于多巴胺针剂和肾上腺素针剂的同时检测.The citric acid modified glassy carbon electrode（CA/GC） was prepared by potentiostatic technique and was used for determinations of dopamine（DA）,epinephrine（EP）,and their mixtures by differential pulse voltammetry（DPV）.The modified electrode shows the enhanced sensitivity and excellent electrochemical discrimination to DA and EP.The cathodic potential difference between DA and EP was about 380 mV,while no cathodic reduction of ascorbic acid（AA） was observed because the oxidation of AA is an irreversible reaction at the sensor.As a result,DA and EP could be determined simultaneously with the interferences of high concentrations of AA being eliminated.The reduction peak currents obtained in the phosphate buffer solutions at pH=6.0 were linearly dependent on the DA and EP concentrations in the ranges of 1.0×10-6~6.0×-5 mol·L-1 and 2.0×10-6~6.0×10-5 mol·L-1,respectively.The proposed method showed potential applications in the simultaneous detections of DA and EP in injections.

关 键 词：修饰电极 柠檬酸 多巴胺 肾上腺素 

分 类 号：R927[医药卫生—药学] O657.1[理学—分析化学]