重楼药材中4种重楼皂苷含量测定的对照品替代方法研究  

Analysis of four saponins in Rhizoma Paridis with substitution method of reference substance

作　　者：曹恒涛[1,2] 鲁静[3] 林瑞超[3] 赵怀清[1] 

机构地区：[1]沈阳药科大学,沈阳110016 [2]安阳市食品药品检验所,安阳455000 [3]中国药品生物制品检定所,北京100050

出　　处：《药物分析杂志》2011年第9期1641-1645,共5页Chinese Journal of Pharmaceutical Analysis

基　　金：国家中医药管理局中药材多基源品种的质量等同性研究,项目编号200707007

摘　　要：目的:建立用替代对照品法测定重楼药材中重楼皂苷Ⅰ、重楼皂苷Ⅱ、重楼皂苷Ⅵ、重楼皂苷Ⅶ4种重楼皂苷的含量测定方法。方法:采用高效液相色谱法,以柴胡皂苷d为替代对照品,使用Waters SymmetryShield RP18(4.6 mm×250 mm,5μm)色谱柱,以乙腈-水为流动相梯度洗脱,流速1.0 mL.min-1,检测波长203 nm,柱温27℃,同时测定重楼药材中4种重楼皂苷的含量。结果:以柴胡皂苷d与4种重楼皂苷的峰面积比值和柴胡皂苷d与4种重楼皂苷的浓度比值得到的标准曲线具有良好的线性关系,加样回收率在101.6%～104.2%之间。结论:柴胡皂苷d作为替代对照品可同时测定重楼药材中重楼皂苷Ⅰ、重楼皂苷Ⅱ、重楼皂苷Ⅵ、重楼皂苷Ⅶ4种重楼皂苷的含量。Objective：To establish a quantitative analysis method of four saponins（Chonglou saponin Ⅰ,Chonglou saponin Ⅱ,Chonglou saponin Ⅵ,Chonglou saponin Ⅶ）in Rhizoma Paridis with a substitute standard.Methods：An HPLC method was builded to determine the four saponins while saikosaponin d was used as substitute standard.The method was established by an Waters SymmetryShield RP18（4.6 mm×250 mm,5 μm）column with 27 ℃ column temperature,the mobile phase of acetonitrile-water with the gradient elution at a flow rate of 1.0 mL·min-1,and the detection wavelength of 203 nm.Results：The four calibration curves which were made by the ratio of peak areas and concentrations were in good linearities,and the recovery was between 101.6% and 104.2%.Conclusion：Saikosaponin d can be used as the substitute standard to determinate the four saponins in Rhizoma Paridis simultaneously.

关 键 词：重楼 重楼皂苷 替代对照品 柴胡皂苷D 含量测定 高效液相色谱 方法学 

分 类 号：R917[医药卫生—药物分析学]