UPLC法测定小叶黑柴胡的根中柴胡皂苷a、d含量  

UPLC determination of saikosaponin a,d in roots of Bupleurum smithii Wolff var.parvifoliaum Shan et Y.Li

作　　者：汤芳玲[1,2] 付珣[1,2] 朱珍真[1] 蔡光明[1] 袁波[2] 张卓勇[3] 

机构地区：[1]中国人民解放军第302医院全军中药研究所,北京100039 [2]沈阳药科大学药学院,沈阳110016 [3]首都师范大学,北京100048

出　　处：《药物分析杂志》2011年第9期1654-1657,共4页Chinese Journal of Pharmaceutical Analysis

基　　金：北京市政府专项基金项目(No:0853279)

摘　　要：目的:建立超高效液相色谱法检测小叶黑柴胡的根中柴胡皂苷a、d含量。方法:采用Waters Acquity UPLC系统,使用ACQUITY UPLC BEHTM C18(50 mm×2.1 mm,1.7μm)色谱柱,流动相为乙腈-水,梯度洗脱,流速0.6 mL.min-1,检测波长203 nm,柱温30℃。结果:柴胡皂苷a线性范围为0.059～1.180μg(r=0.9998),平均回收率为96.7%(RSD=1.3%,n=6);柴胡皂苷d线性范围为0.112～2.230μg(r=0.9997),平均回收率为98.7%(RSD=1.5%,n=6)。结论:该方法操作简便,结果准确,可用于小叶黑柴胡的质量控制。Objective：To establish an UPLC method with Photo Diode Array for simultaneous determination of saikosaponin a,saikosaponin d in roots of Bupleurum smithii Wolff var.parvifoliaum Shan et Y.Li.Methods：The analyses were performed on a Waters Acquity UPLC system,an ACQUITY UPLC BEHTM C18（50 mm×2.1 mm,1.7 μm） column was adopted,the mobile phase consisted of acetonitrile-water with gradient elution at the flow rate of 0.6 mL·min-1,the detection wavelength was set at 203 nm,and the column temperature was 30 ℃.Results：The linear ranges for saikosaponin a,saikosaponin d were 0.059-1.180 μg（r=0.9998） and 0.112-2.230 μg（r=0.9997）,with their average recoveries（n=6） of 96.7%（RSD=1.3%） and 98.7%（RSD=1.5%）,respectively.Conclusion：The method is simple,rapid and accurate,and can be used for the quality control of Bupleurum smithii Wolff var.parvifoliaum Shan et Y.Li.

关 键 词：柴胡 柴胡皂苷A 柴胡皂苷D 超高效液相色谱法 含量测定 

分 类 号：R917[医药卫生—药物分析学]