多波长高效液相色谱法同时测定栀子厚朴汤醇提液中6个主要成分的含量  

Simultaneous determination of six main components in Zhizihoupu ethanol extraction by HPLC at multiple UV wavelengths

作　　者：宋静[1] 于霄[1] 熊志立[1] 李发美[1] 

机构地区：[1]沈阳药科大学药学院,沈阳110016

出　　处：《药物分析杂志》2011年第9期1664-1667,共4页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:建立同时测定栀子厚朴汤醇提液中栀子苷、柚皮苷、橙皮苷、新橙皮苷、和厚朴酚与厚朴酚含量的高效液相色谱方法。方法:采用Hypersil C18(250 mm×4.6 mm,5μm)色谱柱,以乙腈-水为流动相,梯度洗脱,流速1.0 mL.min-1,检测波长为238 nm(0～12 min)、283 nm(12～30 min)、294 nm(30～40 min),柱温30℃。结果:栀子苷、柚皮苷、橙皮苷、新橙皮苷、和厚朴酚、厚朴酚的进样量分别在1.03～10.3μg(r=0.9996)、0.184～1.84μg(r=0.9996)、0.880～8.80μg(r=0.9997)、0.478～4.78μg(r=0.9996)、0.221～2.21μg(r=0.9998)、0.538～5.38μg(r=0.9998)范围内与各自峰面积值呈良好线性关系;平均回收率(n=6)分别为99.4%,99.2%,102.4%,100.3%,102.0%,99.8%。结论:本方法简便、准确、可靠,可用于栀子厚朴汤醇提液的质量控制。Objective：To establish an HPLC method for simultaneous determination of geniposide,naringin,hesperidin,neohesperidin,honokiol,and magnolol in Zhizihoupu ethanol extraction.Methods：Hypersil C18 colunm（250 mm×4.6 mm,5 μm） was adopted with the mobile phase consisting of acetonitrile-water at a flow rate of 1.0 mL·min-1,and the detection wavelength was set at 238 nm（0-12 min）、283 nm（12-30 min）、294 nm（30-40 min）,and the column temperature was maintained at 30 ℃.Results：The calibration curves of geniposide,naringin,hesperidin,neohesperidin,honokiol,and magnolol were in good linearity over the ranges of 1.03-10.3 μg（r=0.9996）,0.184-1.84 μg（r=0.9996）,0.880-8.80 μg（r=0.9997）,0.478-4.78 μg（r=0.9996）,0.221-2.21 μg（r=0.9998）,0.538-5.38 μg（r=0.9998）,respectively.And the average recoveries of the six constituents were 99.4%,99.2%,102.4%,100.3%,102.0% and 99.8%,respectively.Conclusion：The method is simple,accurate and reliable,and can be used for the quality control of Zhizihoupu ethanol extraction.

关 键 词：中药 汤剂 栀子厚朴汤 栀子苷 柚皮苷 橙皮苷 新橙皮苷 和厚朴酚 厚朴酚 高效液相色谱法 

分 类 号：R917[医药卫生—药物分析学]