HPLC法测定复方红景天胶囊中红景天苷和紫丁香苷的含量  

HPLC determination of salidroside and syringin in compound Hongjingtian capsules

作　　者：傅欣彤[1] 王京辉[1] 李琰[1] 张小茜[1] 

机构地区：[1]北京市药品检验所,北京100035

出　　处：《药物分析杂志》2011年第9期1781-1784,共4页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:建立测定复方红景天胶囊中红景天苷和紫丁香苷含量的HPLC法。方法:采用Phenomenex C18(250 mm×4.6mm,10μm)色谱柱测定红景天苷的含量,流动相为甲醇-水(10∶55),流速1.0 mL.min-1,检测波长225 nm,柱温30℃;采用DiscoveryC18(250 mm×4.6 mm,5μm)色谱柱测定紫丁香苷的含量,流动相为乙腈(A)-水(B),梯度洗脱[0～35 min,A-B(6∶94);35～50 min,A-B(60∶40);50～60 min,A-B(6∶94)],流速1.0 mL.min-1,检测波长265 nm,柱温35℃。结果:红景天苷进样量在0.115～0.920μg(r=0.9999)范围内线性关系良好,平均回收率(n=5)为98.53%(RSD=1.1%);紫丁香苷进样量在0.0643～0.515μg(r=0.9999)范围内线性关系良好,平均回收率(n=5)为99.06%(RSD=0.74%)。结论:本方法操作简便,结果准确可靠,重复性好。Objective：To establish an HPLC method for the determination of salidroside and syringin in compound Hongjingtian capsules.Methods：For the determination of salidroside,Phenomenex C18（250 mm×4.6 mm,10 μm） column was adopted,the mobile phase was methanol-water（10∶55） at the flow rate of 1.0 mL·min-1,the detection wavelength was 225 nm and the column temperature was controlled about 30 ℃;For the determination of syringin,Discovery C18（250 mm×4.6 mm,5 μm） column was adopted,the mobile phase was acetonitrile（A）-water（B） with gradient elution[A-B（6∶94） in 0-35 min,A-B（60∶40） in 35-50 min,A-B（6∶94） in 50-60 min] at the flow rate of 1.0 mL·min-1,the detection wavelength was 225 nm,and the column temperature was controlled about 35 ℃.Results：For salidroside,the method had good linear relationship within the range of 0.115 to 0.920 μg with the correlation coefficients 0.9999,the average recovery was 98.53% with RSD of 1.1%;For syringin,the method had good linear relationship within the range of 0.0643 to 0.515 μg with the correlation coefficients 0.9999,the average recovery was 99.06% with RSD of 0.74%.Conclusion：The method is simple,accurate,sensitive and has good repetition.

关 键 词：中药 复方红景天胶囊 红景天苷 紫丁香苷 高效液相色谱法 

分 类 号：R917[医药卫生—药物分析学]