引种紫锥菊中菊苣酸的定性定量分析  

Determination of cichoric acid in introducing plant of Echinacea purpurea

作　　者：李晶[1] 聂斌[2] 武力[2] 曾振灵[1] 吴鸿[3] 陈建新[1] 

机构地区：[1]华南农业大学兽医学院,广州510642 [2]华南农业大学实验兽药厂,广州510642 [3]华南农业大学药用植物研究中心,广州510642

出　　处：《药物分析杂志》2011年第9期1804-1807,共4页Chinese Journal of Pharmaceutical Analysis

基　　金：广东省教育部产学研合作专项资金项目(2008B090500250)

摘　　要：目的:建立薄层色谱法(TLC)和高效液相色谱法(HPLC),对紫锥菊不同部位中的菊苣酸进行分析,为紫锥菊质量标准的建立提供依据。方法:TLC鉴别紫锥菊根、茎、叶、花中的菊苣酸;HPLC测定以上各部位中菊苣酸的含量,色谱柱为XTerraC18柱(150 mm×4.6 mm,5μm),流动相为乙腈-0.1%磷酸水溶液(23︰77),流速为1.0 mL.min-1,紫外检测波长为326 nm。结果:TLC中菊苣酸的斑点清晰可辨,Rf值为0.72;HPLC测得菊苣酸的进样浓度在0.5～100μg.mL-1范围内与峰面积呈良好的线性关系(r=0.9999),方法的平均回收率(n=3)为95.3%(RSD=3.6%),紫锥菊根、茎、叶、花中的菊苣酸含量分别为12.1,0.68,5.60,2.47 mg.g-1。结论:该检测方法简便可行,可用于紫锥菊中菊苣酸的质量控制。Objective：To establish TLC and HPLC methods to analyze cichoric acid in different parts of Echinacea purpurea.Methods：TLC was adopted to rapidly identify the cichoric acid of different parts of Echinacea purpurea,including root,stem,leaf and flower,and HPLC was adopted to determine the content of cichoric acid in different parts of the plant.The column of XTerra C18（150 mm×4.6 mm,5 μm）was adopted,with mixture of acetonitrile-0.1% phosphoric acid（23︰77）as mobile phase at flow rate of 1.0 mL·min-1,and the UV detector was set at 326 nm.Results：The cichoric acid dot of TLC was clear and the Rf value was 7.2.For HPLC determination,a good linear relationship in the range of 0.5-100 μg·mL-1（r=0.9999）and ideal average recovery（95.3%,RSD=3.6%,n=3）were obtained.The content of the cichoric acid in root,stem,leaf and flower of Echinacea purpurea was 12.1,0.68,5.60,2.47 mg·g-1 respectively.Conclusion：The method is simple,and can be used to control quality of cichoric acid in Echinacea purpurea.

关 键 词：紫锥菊(紫花松果菊) 根 茎 叶 花 菊苣酸 薄层色谱法 高效液相色谱法 

分 类 号：R917[医药卫生—药物分析学]