星点设计-效应面法优选暴马丁香树皮中紫丁香苷的提取工艺  

Optimization of extraction process based on central composite design and response surface methodology for syringin in the peels of Syringa reticulata

作　　者：蔡恩博[1] 张崇禧[2] 朱启光[1] 吴谦[1] 徐蕊[1] 李鹏宇[1] 郑友兰[1] 

机构地区：[1]吉林农业大学中药材学院,长春130118 [2]山东大学威海分校海洋学院,威海264209

出　　处：《药物分析杂志》2011年第9期1808-1811,共4页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:优选暴马丁香树皮中紫丁香苷的提取工艺。方法:采用星点设计-效应面法,以乙醇浓度、提取时间、溶媒比为主要影响因素,紫丁香苷提取率为评价指标,通过对各因素的多元线性回归及二项式拟合,采用效应面法和岭嵴分析优选最佳提取工艺,并进行预测分析。结果:确定紫丁香苷最佳提取工艺为46%乙醇20倍量超声提取85 min。结论:星点设计–效应面法优选暴马丁香树皮中紫丁香苷的提取工艺,实际值与预测值吻合度高,预测性良好,方法简便易行,为更好地开发利用暴马丁香树皮中紫丁香苷提供参考依据。Objective：To optimize the extraction process for syringin in peels of Syringa reticulata by central composite design and response surface methodology.Method：Central composite design and response surface methodology was used to optimize the extracting process.The main influential factors of extraction efficiency are ethanol concentration,reflux time,and solvent ratio.The conditions of the extraction were evaluated by the total content of syringin.Multiple linear regression and binomial fitting models were performed with the main influential factors.A two-order polynomial equation was fitted to the data and resultant model was used to predict the response in the optimal region.Result：Analysis indicated the optimum conditions of syringin are 46% ethanol,20 fold solvent,ultrasonic extraction 85 minutes.Conclusion：It shows that the optimum process for extracting the syringin in the peels of Syringa reticulata is simple,more convenient and with an higher precision.The procedure developed in this study provides a theoretical basis for better development and utilization syringe.

关 键 词：暴马丁香(白丁香) 紫丁香苷 星点设计 效应面法 工艺设计 制药工艺学 

分 类 号：R917[医药卫生—药物分析学]