Production of CCHF Virus-Like Particle by a Baculovirus-Insect Cell Expression System  

作　　者：Zhao-rui Zhou Man-li Wang Fei Deng Tian-xian Li Zhi-hong Hu Hua-lin Wang 

机构地区：[1]State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China

出　　处：《Virologica Sinica》2011年第5期338-346,共9页中国病毒学（英文版）

基　　金：The Knowledge Innovation Program of the Chinese Academy of Sciences,Grant No.KSCX2-YW-N-065;the Knowledge Innovation Program of the Chinese Academy of Sciences,Grant No.KSCX2-EW-G-8;National Key Basic Research Program(973Program),Grant No.2010CB530103;National Basic Research Priorities Program of China,Grant No.2007FY210700

摘　　要：Crimean-Congo Haemorrhagic Fever Virus (CCHFV) is a tick-born virus of the Nairovirus genus within the Bunyaviridae family,which is widespread and causes high fatality.The nucleocapsid of CCHFV is comprised of N proteins that are encoded by the S segment.In this research,the N protein of CCHFV was expressed in insect cells using a recombinant baculovirus.Under an electron microscope,Virus-Like Particles (VLPs) with various size and morphology were observed in cytoplasmic vesicles in the infected cells.Sucrose-gradient purification of the cell lysate indicated that the VLPs were mainly located in the upper fraction after ultracentrifugation,which was confirmed by Western blot analysis and immuno-electron microscopy (IEM).Crimean-Congo Haemorrhagic Fever Virus （CCHFV） is a tick-born virus of the Nairovirus genus within the Bunyaviridae family, which is widespread and causes high fatality. The nucleocapsid of CCHFV is comprised of N proteins that are encoded by the S segment. In this research, the N protein of CCHFV was expressed in insect cells using a recombinant baculovirus. Under an electron microscope, Virus-Like Particles （VLPs） with various size and morphology were observed in cytoplasmic vesicles in the infected cells. Sucrose-gradient purification of the cell lysate indicated that the VLPs were mainly located in the upper fraction after ultracentrifugation, which was confirmed by Western blot analysis and immuno-electron microscopy （IEM）

关 键 词：Crimean-Congo hemorrhagic fever virus （CCHFV） Virus-Like Particle （VLP） Nucleocapsid （N）protein 

分 类 号：S435.111.4[农业科学—农业昆虫与害虫防治] TQ453.5[农业科学—植物保护]