陆地棉质体转录活性因子基因GhPTAC的克隆及其耐盐性分析  

Cloning and Salt-tolerance Analysis of Gene Plastid Transcriptionally Active(GhPTAC) from Gossypium hirsutum L.

作　　者：周凯[1] 叶武威[1] 王俊娟[1] 王德龙[1] 樊保香[1] 王帅[1] 

机构地区：[1]中国农业科学院棉花研究所,棉花生物学国家重点实验室,农业部棉花遗传改良重点实验室,河南安阳455000

出　　处：《作物学报》2011年第9期1551-1558,共8页Acta Agronomica Sinica

基　　金：国家转基因生物新品种培育科技重大专项(2008ZX08005-004);国家“十一五”科技支撑计划项目(2006BAD13B04-1)资助

摘　　要：为了挖掘新的耐盐基因及调控途径,利用基因芯片技术及抑制性差减文库技术筛选到质体转录活性因子,通过RACE及RT-PCR技术克隆到该基因的cDNA全长,命名为GhPTAC。该cDNA全长1564bp,其中ORF1038bp,推测编码345个氨基酸残基的多肽。生物信息学分析表明GhPTAC为拟南芥PTAC13同源基因,同源性60.6%。编码蛋白为转录活跃的染色体(TAC)的一个组分,参与叶绿体基因组转录终止/抗终止调节。real-timePCR分析结果表明,GhPTAC受盐胁迫诱导上调表达,在耐盐材料中9806中表达水平明显高于盐敏感材料中S9612,这与芯片结果一致。To develop novel salt-tolerance genes and adjustment pathway,we screened out gene Plastid Transcriptionally Active named as GhPTAC based on salt-tolerance gene chips and salt resistance related SSH library.The result of RACE and RT-PCR showed that the cDNA full length was 1 564 bp,and ORF was 1 038 bp,which encoded 345 amino acid residues.Bioinformatics analysis showed that GhPTAC shared the identity of 60.6% with the homologous gene PTAC13 from Arabidopsis thaliana.As one part of Transcriptionally active chromosome（TAC）,GhPTAC plays a part role in regulation of transcription termination/antitermination of chloroplastid genome.The GhPTAC expression was up-regulated under salt stress induction and the expression level of GhPTAC of Zhong 9806（salt-resistant material） was obviously higher than that Zhong S9612（salt-sensitive material） which was measured by real-time PCR and in accord with the arrays results.

关 键 词：陆地棉 盐胁迫 PTAC real—time PCR 

分 类 号：S562[农业科学—作物学]