托佩克猪SLA-2基因克隆及功能区生物信息学分析  

Cloning of the SLA-2 Gene from TOPIGS Pig and Analysis Its Functional Domain by Bioinformatics

作　　者：白婧[1] 许崇波[1] 张强[2] 高凤山[1,2] 

机构地区：[1]大连大学生物工程学院生物化学和分子生物学实验室,大连116622 [2]中国农业科学院兰州兽医研究所家畜疫病病原微生物国家重点实验室,兰州730046

出　　处：《生物技术通报》2011年第8期133-139,共7页Biotechnology Bulletin

基　　金：国家自然科学基金项目(30972169);家畜疫病病原生物学国家重点实验室开放基金项目(SKLVEB2009KFKT007)

摘　　要：为研究托佩克猪SLA-2基因功能,设计引物,克隆了该品系猪SLA-2基因(SLA-2-TPK)cDNA全长序列,并通过分子生物学软件分析其基因特征。结果显示,SLA-2-TPK cDNA全长为1 119 bp,其中3-1 097为编码区,共编码364个氨基酸,分别在第125、188、227和283位置出现半胱氨酸残基,含有两对链内二硫键。氨基酸同源性分析显示,SLA-2-TPK与其他SLA-2、SLA-3和SLA-1序列的同源率分别为86.0%-98.9%、85.0%-89.4%和84.2%-91.7%。系统进化树显示,SLA-2-TPK与韩国品系猪DQ883211遗传距离较近;各功能区分析,SLA-2-TPK与人HLA-A2和小鼠H-2K分子结构相似,且保留了人HLA-A2基因的部分功能位点。结果表明,SLA-2-TPK是典型的SLA-2等位基因,在进化程度上接近于部分韩国品系猪。In order to study the function of SLA-2 gene for TOPIGS pig,a pair of primers were designed to amplify the whole cDNA of SLA-2 from TPPIGS pig and then the genetic characterization of the interest gene was analyzed by computer.After cloning,sequencing and analyzing by computer,SLA-2-TPK was 1 119 bp and the 3-1 097 site was the ORF domain which coded for 364 amino acids with two sets of disulfide bond in intro-chain constituted by four cysteines situated in 125,188,227 and 283 sites.The amino acid identical ratios between SLA-2-TPK and other SLA-2,SLA-3 and SLA-1 alleles were 86.0%-98.9%,85.0%-89.4% and 84.2%-91.7%,respectively.The phylogenetic tree of the SLA-2-TPK demonstrated that it was close to some breeds of swine in Korean,such as DQ883211.Besides,the SLA-2-TPK was similar to the HLA-A2 and H-2K in functional domains and it preserved some functional sites of HLA-A2.The results indicated that SLA-2-TPK was a typical SLA-2 allele that might be closely relative to some breeds of swine in Korean.

关 键 词：托佩克猪 SLA-2基因 克隆 功能区 生物信息学 

分 类 号：S828[农业科学—畜牧学] Q78[农业科学—畜牧兽医]