悬浮芯片技术应用进展  

Application Advance of Suspension Array Technology

作　　者：程涛[1,2] 王慧煜[1] 梅琳[1] 韩雪清[1] 

机构地区：[1]中国检验检疫科学研究院,北京100029 [2]南京师范大学生命科学学院,南京210046

出　　处：《生物技术通报》2011年第9期48-51,58,共5页Biotechnology Bulletin

基　　金：国家转基因重大专项项目(2008ZX08012-001);质检公益性行业科研专项(200910132)

摘　　要：悬浮芯片技术(SAT)是一种新型、高通量的生物芯片技术,它是将流式细胞术、激光技术及应用流体学等技术结合在一起,利用悬浮在液相中的分类荧光编码微球作为检测载体,具有高通量、速度快、灵敏度高、特异性强及检测范围广等特点。近几年来,悬浮芯片技术在免疫学、基因组学、蛋白质组学及临床诊断检测等方面应用较广泛。就其原理、技术特点和应用作一介绍。Suspension array technology（SAT）is a new and high-throughput microarray technology.It integrates flow cytometry,laser technology,fluidics and other technologies.The classification fluorescent encoded microspheres,which suspended in the liquid phase are used as carrier of detection.The benefits of SAT include high-throughput,rapidness,excellent sensitivity and specificity,detection of a wide range.In recent years,SAT is being used in a variety of applications,such as immunology,genomics,proteomics,clinical diagnostic tests etc.This article intended to provide a comprehensive review of its principles,technical characteristics,and reviewed applications in protein and nucleic acid detection.

关 键 词：生物芯片 悬浮芯片技术(SAT) 蛋白检测 核酸检测 高通量 

分 类 号：Q503[生物学—生物化学]