宏基因组技术在开发极端环境未培养微生物中的应用  

Application of Metagenomic Technique in the Exploring of Uncultured Microbial in Extreme Environments

作　　者：朱允华[1,2,3] 李俭[1,2] 方俊[1,2] 田云[1,2] 卢向阳[1,2] 

机构地区：[1]湖南农业大学生物科学技术学院,长沙410128 [2]湖南省农业生物工程研究所,长沙410128 [3]南华大学药学与生命科学学院,衡阳421001

出　　处：《生物技术通报》2011年第9期52-58,共7页Biotechnology Bulletin

基　　金：国家"十一五"科技支撑计划(2008BADC4B08);科技部国际科技合作项目(S2010GR0966)

摘　　要：人类对自然环境中约99%的微生物不能用传统的方法进行纯培养,对于极端环境中的微生物更是知之甚少。随着宏基因组技术的出现,人们可对这一庞大的未知世界进行多方面研究。目前研究者利用这一技术已经对地球上的多种极端生境进行了研究,并取得了很多新的成就。简要概述这一技术在极端环境未培养微生物研究中的应用。It is known that about 99% microorganisms in natural environment couldn＇t be obtained their pure culture by traditional culture methods,but for extremophiles of extreme conditions is poorly understood.With the emergence of metagenomic technology,people could investigate the immense and unknown world of microorganisms in many ways.Investigators have studied many extreme habitats of the earth by this technology and acquired many novel accomplishments.The application of metagenomics for investigation of uncultured microorganisms in extreme environments has been briefly summarized in this paper.

关 键 词：宏基因组技术 极端环境 未培养微生物 

分 类 号：Q93[生物学—微生物学]