微滴培养技术的新应用  

The New Applications of Micro-drop Culture Technique

作　　者：霍龙[1] 张岩[2] 吴应积[1,2] 

机构地区：[1]内蒙古大学生命科学学院,呼和浩特010021 [2]内蒙古大学哺乳动物生殖生物学与生物技术教育部重点实验室,呼和浩特010021

出　　处：《生物技术通报》2011年第9期59-62,共4页Biotechnology Bulletin

基　　金：国家大学生创新性实验计划(101012610);高等学校博士学科重点专项科研基金项目(20101501110001)

摘　　要：微滴培养技术在卵母细胞培养和胚胎早期发育研究中有广泛的应用。近期研究发现这项技术又有新的应用范围。有科学家发现将该技术应用于胚胎干细胞可以实现高效传代,在精原干细胞培养和精子发生相关过程的研究方面也可取得很好效果,同时,对早期人类胚胎干细胞的分离过程的监控和对精原干细胞生长过程的观察也相对容易。这些研究结果显示,微滴培养技术在这些新领域的研究与常规培养方法相比具有独特的优势。回顾微滴培养技术的主要发展过程,重点探讨微滴培养技术的最新应用及其优点。Micro-drop culture technique has been used widely in the oocyte culture and the development of early embryos.It has been found this technology has multiple new applications by recent studies.Some scientists have found that this technique used in embryonic stem cells can achieve high efficiency in passage,used in in vitro culture of spermatogonial stem cells and related spermatogenesis can be also very desirable,while it＇s relatively easier to monitor the derivation of the early human embryonic stem cells and observe the process of the growth of spermatogonial stem cells.These results showed that micro-drop culture technique in these new areas of research has multiple special advantages compared with the conventional culture methods.In this review,we describe the development process of the micro-drop culture technique,and focus on the latest applications and advantages of this technique.

关 键 词：微滴培养技术 新应用 体外培养 胚胎干细胞 精原干细胞 

分 类 号：Q813[生物学—生物工程]