草莓果实MADS-box基因保守片段的克隆与序列分析  

Cloning and Sequences Analysis of MADS-box Genes Fragments from Strawberry(Fragaria ananassa Duch.)Fruit

作　　者：康兆茹[1,2] 周厚成[2] 赵霞[2] 张敏[1] 李凌[1] 

机构地区：[1]西南大学园艺园林学院,重庆400715 [2]中国农业科学院郑州果树研究所,郑州450009

出　　处：《生物技术通报》2011年第9期76-80,89,共6页Biotechnology Bulletin

基　　金：河南省重点科技攻关项目(092102110038);中国农业科学院科技经费项目(2009)

摘　　要：根据多种植物MADS-box基因保守区序列设计简并引物,应用PCR技术从草莓绿果中分离出33条MADS-box基因cDNA片段。序列分析表明,这些片段长度在137-146 bp之间,包含基因起始密码子。推导的氨基酸序列与已登录的草莓的MADS-box基因同源性超过87%。推导的氨基酸序列与已知的草莓和其他物种调控果实发育成熟的MADS-box基因以及拟南芥的MADS-box家族基因进行系统发育分析,可将这些基因片段分别归入拟南芥MADS-box基因不同亚家族中,证明草莓果实中存在各类MADS-box家族基因,克隆的部分片段可能参与调控果实发育和成熟软化的调节。According to the conservative region sequences of MADS-box family genes from various plants,we designed degenerate primers for obtaining 33 MADS-box gene fragments by RT-PCR from strawberry green fruit total RNA.Sequence analysis indicated the length of these fragments was between 137-146 bp,including start codon of genes,deduced amino acid sequences had a higher identity of over 87%with MADS-box genes of strawberry fruit.All these gene fragments were divided into the Arabidopsis MADS-box gene subfamily by phylogeny construction of sequences.These results indicated that there were many MADS-box genes in strawberry.Some of these gene fragments may play an important role in adjusting fruit development,ripening even to softening.

关 键 词：草莓 MADS-BOX基因 RT-PCR 序列分析 

分 类 号：S668.4[农业科学—果树学]